anti-Human Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Dot Blot

Recommended Microtubule-Associated Protein 1 Light Chain 3 beta Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Epitope
pThr12
86
51
23
21
20
17
10
10
9
8
8
6
6
5
5
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
267
158
110
32
27
27
26
25
12
12
6
6
1
Host
Rabbit
250
20
1
Clonality
Polyclonal
Conjugate
Un-conjugated
18
15
15
14
13
13
6
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB)
159
121
91
75
73
68
62
38
26
10
9
6
6
3
2
1
1
1
Options
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Catalog No. ABIN389913
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.633324 ABIN1449629 DB EIA Rabbit Ig AA 1-30, pThr12, N-Term Log in to see Polyclonal 8
29.633324 ABIN3031788 DB ELISA Rabbit Ig Fraction pThr29 Log in to see Polyclonal
29.633324 ABIN3031787 DB ELISA Rabbit Ig Fraction pThr12 Log in to see Polyclonal
29.633324 ABIN3028838 DB ELISA Rabbit Ig Fraction pThr93, pTyr99 Log in to see Polyclonal
1 ABIN1953095 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr29 Log in to see Polyclonal
1 ABIN1953967 DB ELISA APC Rabbit IgG Thr29 Log in to see Polyclonal
1 ABIN1955188 DB ELISA Biotin Rabbit IgG pThr12 Log in to see Polyclonal
1 ABIN1956446 DB ELISA FITC Rabbit IgG Thr93, Tyr99 Log in to see Polyclonal
1 ABIN1957644 DB ELISA HRP Rabbit IgG pThr93 Log in to see Polyclonal
1 ABIN1957566 DB ELISA HRP Rabbit IgG pThr12 Log in to see Polyclonal
1 ABIN1956364 DB ELISA FITC Rabbit IgG pThr12 Log in to see Polyclonal
1 ABIN1956404 DB ELISA FITC Rabbit IgG Thr29 Log in to see Polyclonal
1 ABIN1956445 DB ELISA FITC Rabbit IgG pThr93 Log in to see Polyclonal
1 ABIN1958740 DB ELISA PE Rabbit IgG Thr29 Log in to see Polyclonal
1 ABIN1958696 DB ELISA PE Rabbit IgG pThr12 Log in to see Polyclonal
1 ABIN1958784 DB ELISA PE Rabbit IgG Thr93, Tyr99 Log in to see Polyclonal
1 ABIN1955266 DB ELISA Biotin Rabbit IgG pThr93 Log in to see Polyclonal
1 ABIN1953923 DB ELISA APC Rabbit IgG pThr12 Log in to see Polyclonal
1 ABIN1953138 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr93 Log in to see Polyclonal
1 ABIN1953139 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr93, Tyr99 Log in to see Polyclonal

Top referenced anti-Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Dot Blot

Similar anti-Microtubule-Associated Protein 1 Light Chain 3 beta Antibodies

Application / Reactivity Human
Dot Blot (DB) 76 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 119 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 38 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 73 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 91 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 10 Antibodies
Immunohistochemistry (IHC) 67 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 6 Antibodies
Western Blotting (WB) 154 Antibodies

General

Antigen Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
Epitope pThr12
(86), (51), (23), (21), (20), (17), (10), (10), (9), (8), (8), (6), (6), (5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(267), (158), (110), (32), (27), (27), (26), (25), (12), (12), (6), (6), (1)
Host Rabbit
(250), (20), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate Un-conjugated
(18), (15), (15), (14), (13), (13), (6), (5), (5), (5), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(159), (121), (91), (75), (73), (68), (62), (38), (26), (10), (9), (6), (6), (3), (2), (1), (1), (1)
Pubmed 6 references available
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Product details

Target details Application Details Handling References Images
Specificity This Phospho-LC3B-T12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding T12 of human LC3 (APG8b). Patent pending.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This LC3B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B.
Clone RB12679
Isotype Ig

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name LC3B (MAP1LC3B Antibody Abstract)
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 DA
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Autophagy, Cell Structure
Pathways Autophagy

Application Details

Product details Target details Handling References Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References

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Product cited in:

Mitroulis, Kourtzelis, Kambas, Rafail, Chrysanthopoulou, Speletas, Ritis: "Regulation of the autophagic machinery in human neutrophils." in: European journal of immunology, Vol. 40, Issue 5, pp. 1461-72, 2010

Background publications

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004

He, Dang, Dai, Guo, Wu, She, Pei, Chen, Ling, Wu, Zhao, Liu, Yu: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003

Images

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Supplier Images
Dot Blot (DB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody (ABIN389913) Dot blot analysis of Phospho-LC3 (APG8b)- T12 Antibody (ABIN389913) on nitrocellulose...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody (ABIN389913) Immunofluorescence confocal microscopy staining of LC3B. (I) Control cells treated wi...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody (ABIN389913) (Left)Formation of LC3B puncta in phagocytosing neutrophils and(Right) in cells treat...