anti-Mouse (Murine) Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Immunofluorescence

Recommended Microtubule-Associated Protein 1 Light Chain 3 beta Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Epitope
N-Term
85
51
28
21
21
17
15
14
10
9
8
6
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish
270
157
109
31
26
26
25
24
12
12
6
6
1
Host
Rabbit
253
20
1
Clonality
Polyclonal
Conjugate
Un-conjugated
18
15
15
14
13
13
6
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
159
121
92
76
73
67
62
38
25
10
9
6
5
3
2
1
1
Supplier
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Catalog No. ABIN153145
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.178295 ABIN250638 ELISA ICC IF IHC IHC (fro) IHC (p) IP PLA SimWes WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal 592
11.178295 ABIN492614 IF WB Mouse IgG1 N-Term Log in to see 5F10
11.178295 ABIN1449601 EIA IF Rabbit Ig C-Term, AA 96-125, cleaved Log in to see Polyclonal 11
11.178295 ABIN4330478 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-100, N-Term Log in to see 1251A 2
11.178295 ABIN3198184 IC IF IHC WB Rabbit Log in to see Polyclonal
11.178295 ABIN3031785 ICC IF IHC ELISA WB Rabbit Ig Fraction AA 89-120 Log in to see Polyclonal
11.178295 ABIN5529644 ICC IF SimWes WB Rabbit IgG AA 1-100, N-Term Log in to see 1251D
11.178295 ABIN3031786 ICC IF ELISA Rabbit Ig Fraction AA 89-122 Log in to see Polyclonal
11.178295 ABIN2563753 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN388484 ICC IF Rabbit Ig AA 89-122, C-Term, cleaved Log in to see Polyclonal 11
1 ABIN250639 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Rabbit N-Term, AA 1-100 Log in to see Polyclonal 4
1 ABIN492615 IF WB Mouse IgG1 N-Term Log in to see 2G6
1 ABIN4330466 FACS ICC IF IHC (fro) IHC (p) FITC Rabbit N-Term Log in to see Polyclonal 1
1 ABIN4330470 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 1-100, N-Term Log in to see Polyclonal 2
1 ABIN4330471 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 1-100, N-Term Log in to see Polyclonal 1
1 ABIN406993 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit N-Term, AA 1-100 Log in to see Polyclonal 2
1 ABIN269556 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit N-Term Log in to see Polyclonal 4
1 ABIN1736444 IEM ICC FACS IF IHC WB Rabbit N-Term Log in to see Polyclonal
1 ABIN492613 IF WB Biotin Mouse IgG1 N-Term Log in to see 5F10
1 ABIN4330463 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 1-100, N-Term Log in to see Polyclonal

General

Antigen Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
Epitope N-Term
(85), (51), (28), (21), (21), (17), (15), (14), (10), (9), (8), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish
(270), (157), (109), (31), (26), (26), (25), (24), (12), (12), (6), (6), (1)
Host Rabbit
(253), (20), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(18), (15), (15), (14), (13), (13), (6), (5), (5), (5), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(159), (121), (92), (76), (73), (67), (62), (38), (25), (10), (9), (6), (5), (3), (2), (1), (1)
Pubmed 20 references available
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Product details

Target details Application Details Handling References Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cytoskeleton, Autophagy, Cancer
Pathways Autophagy

Application Details

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Application Notes Western Blot 1:1000, Simple Western 1:100, Flow Cytometry 1:200, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 0.1-2 μg/mL, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:400, Electron MicroscopyThis LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for LC3B Antibody Fluorescent Staining
1. After washing sample(s) in PBS, fix for 20 minutes at room temperature.
. Wash sample(s) in PBS 3x 3 minutes, each.
. Block sample(s) with 5 % NGS (keep Triton X-100 at 0.1 %) for 60 minutes at room temperature
. Prepare primary anti-LC3B solution during this time at a 1:5,000 dilution with 1 %NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the primary Ab overnight at 4C in a humidified containerNote: If thick tissue samples, Ab must be prepared in 0.5 % Triton X-100/PBSDay
. Wash sample(s) in PBS 3x 5 minutes with wells on. Prepare Alexa-fluor antibodies (Goat anti-Rabbit IgG H+L) as secondary Ab with 1 % NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the appropriate secondary Ab for 1 hour in a dark container.
. Rinse the sample(s) in PBS 4x 10 minutes, each.
. Place one drop of Vectashield onto the slide and cover with a coverslip
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References

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Product cited in:

Liu, Weng, Lin, Tang, Chen, Liang, Ku, Lin: "Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells." in: Scientific reports, Vol. 7, pp. 41437, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kristensen, Oppermann, Rauen, Hartmann-Petersen, Thirstrup: "Polyglutamine expansion of ataxin-3 alters its degree of ubiquitination and phosphorylation at specific sites." in: Neurochemistry international, Vol. 105, pp. 42-50, 2017

Lu, Bradley, McCoski, Gonzalez, Ealy, Johnson: "Reduced skeletal muscle fiber size following caloric restriction is associated with calpain-mediated proteolysis and attenuation of IGF-1 signaling." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 312, Issue 5, pp. R806-R815, 2017

Wang, Cao, Han, Sun, Feng: "Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy." in: Aging and disease, Vol. 8, Issue 1, pp. 71-84, 2017

Tang, Chai, Ye, Yu, Cao, Yang, Xie, Yang: "HMGB1 promotes differentiation syndrome by inducing hyperinflammation via MEK/ERK signaling in acute promyelocytic leukemia cells." in: Oncotarget, Vol. 8, Issue 16, pp. 27314-27327, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sioutas, Vainikka, Kentson, Dam-Larsen, Wennerström, Jacobson, Persson: "Oxidant-induced autophagy and ferritin degradation contribute to epithelial-mesenchymal transition through lysosomal iron." in: Journal of inflammation research, Vol. 10, pp. 29-39, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Zhu, Shui, Liu, Hu, Wang: "Increased autophagic degradation contributes to the neuroprotection of hydrogen sulfide against cerebral ischemia/reperfusion injury." in: Metabolic brain disease, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Hung, Kao, Liu, Huang, Yang, Pan: "Dopamine elevates intracellular zinc concentration in cultured rat embryonic cortical neurons through the cAMP-nitric oxide signaling cascade." in: Molecular and cellular neurosciences, Vol. 82, pp. 35-45, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Pagliassotti, Estrada, Hudson, Wei, Wang, Seals, Zigler, LaRocca: "Trehalose supplementation reduces hepatic endoplasmic reticulum stress and inflammatory signaling in old mice." in: The Journal of nutritional biochemistry, Vol. 45, pp. 15-23, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Saha, Yin, Kim, Yang, Dayem, Choi, Cho: "Valproic Acid Induces Endocytosis-Mediated Doxorubicin Internalization and Shows Synergistic Cytotoxic Effects in Hepatocellular Carcinoma Cells." in: International journal of molecular sciences, Vol. 18, Issue 5, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Gao, Zhuang, Gao, Li, Zhang, Ye, Li, Tang, Zhou, Han, Li: "Tetrahydrocurcumin reduces oxidative stress-induced apoptosis via the mitochondrial apoptotic pathway by modulating autophagy in rats after traumatic brain injury." in: American journal of translational research, Vol. 9, Issue 3, pp. 887-899, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Myeku, Clelland, Emrani, Kukushkin, Yu, Goldberg, Duff: "Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling." in: Nature medicine, Vol. 22, Issue 1, pp. 46-53, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Santanam, Banach-Petrosky, Abate-Shen, Shen, White, DiPaola: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry

Bauckman, Mysorekar: "Ferritinophagy drives uropathogenic Escherichia coli persistence in bladder epithelial cells." in: Autophagy, Vol. 12, Issue 5, pp. 850-63, 2016 (Sample species: Human). Further details: Western Blotting

Alán, Špaček, Pajuelo Reguera, Jabůrek, Ježek: "Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress." in: Toxicology and applied pharmacology, Vol. 302, pp. 31-40, 2016 (Sample species: Human). Further details: Western Blotting

Lee, Du, Stitham, Atteya, Lee, Xiang, Wang, Jin, Leslie, Spollett, Srivastava, Mannam, Ostriker, Martin, Tang, Hwa: "Inducing mitophagy in diabetic platelets protects against severe oxidative stress." in: EMBO molecular medicine, Vol. 8, Issue 7, pp. 779-95, 2016 (Sample species: Human). Further details: Flow Cytometry

Cemma, Grinstein, Brumell: "Autophagy proteins are not universally required for phagosome maturation." in: Autophagy, Vol. 12, Issue 9, pp. 1440-6, 2016 Further details: Western Blotting

Shui, Liu, Zhu, Wang: "Exogenous hydrogen sulfide attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy in mice." in: Canadian journal of physiology and pharmacology, pp. 1-6, 2016

Sousa, Biancur, Wang, Halbrook, Sherman, Zhang, Kremer, Hwang, Witkiewicz, Ying, Asara, Evans, Cantley, Lyssiotis, Kimmelman: "Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion." in: Nature, Vol. 536, Issue 7617, pp. 479-83, 2016 Further details: Western Blotting

Qi, Man, Malireddi, Karki, Lupfer, Gurung, Neale, Guy, Lamkanfi, Kanneganti: "Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2081-97, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using AB...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Staining of treated U373-MG (human glioblastoma) cells using ABIN153145.
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunofluorescent staining of treated U373-MG cells using ABIN153145. The nuclei were...
Simple Western (SimWes) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Simple Western: LC3B/MAP1LC3B Antibody [ABIN153145] - Simple Western lane view shows ...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3B staining in gliobast...
Flow Cytometry (FACS) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Flow Cytometry: LC3B/MAP1LC3B Antibody [ABIN153145] - Staining of NTERA-2 cells using...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3B staining in treated ...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Western Blot: LC3B/MAP1LC3B Antibody [ABIN153145] - analysis of LC3B in IB3-1 whole c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody [ABIN153145] - analysis of U87M...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3 ant...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - IF Conf...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN153145) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - analysi...