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Protein G Resin

Purif, AC Protein G Agarose beads 90 µm
Catalog No. ABIN1536254
  • Target See all Protein G products
    Protein G
    Reactivity
    Streptococcus
    Application
    Purification (Purif), Affinity Chromatography (AC)
    Purpose
    Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. 
    Characteristics
    The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
    Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
    - Number of IgG binding sites per ligand: 3
    - MW of ligand: Approximately 22 kDa
    - PI of ligand: 4.69
    - Degree of substitution: Approximately 2 mg protein G/ml settled resin
    - Static binding capacity: >20 mg sheep IgG/ml settled resin
    - Matrix spherical: agarose, 4% cross-linked
    - Average particle size: 90 μm (45-165 μm)
    Bead Ligand
    Protein G
    Bead Matrix
    Agarose beads
    Bead Size
    90 µm
  • Comment

    High temperature heating is not recommended. The agarose melts above 65°C.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    1X PBS containing 20% ethanol
    Handling Advice
    High temperature heating is not recommended. The agarose melts above 65°C.
    Storage
    4 °C
    Storage Comment
    Store regenerated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
    Expiry Date
    18 months
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    Bikkavilli, Malbon: "Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2." in: Journal of cell science, Vol. 125, Issue Pt 10, pp. 2446-56, (2012) (PubMed).

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  • Target
    Protein G
    Abstract
    Protein G Products
    Background
    Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
    Broad IgG binding spectrum
    Binding specificity complements of protein A
    Agarose media
    No specific albumin binding
    Optimized homogeneous recombinant ligand
    High capacity
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