Protein A Agarose Beads / Resin

Details for Product No. ABIN2039393, Supplier: Log in to see
Antigen
  • alpha protein
  • repB
Reactivity
Staphylococcus aureus (S. aureus)
Application
Separation (Sep), Purification (Purif)
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Purpose Reuseable Ultra-linked rProtein A resin for immunoprecipitation and antibody purification.
Characteristics Protein A Agarose Beads / Resin was produced by immobilizing recombinant protein A to 4% cross-linked agarose matrix. The recombinant protein A contains IgG binding domains, eliminating nonspecific binding sites and reducing steric hindrance.
ProductDetails: Bead Ligand Protein A
ProductDetails: Bead Matrix Agarose beads
ProductDetails: Bead Size 90 µm
Application Notes Column: 5x 45 mm, bed volume 0.9 ml
Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
Comment

Ligand Recombinant protein A
Dynamic binding capacity: ~30 mg human IgG/ml drained medium
Matrix: 4% highly cross-linked agarose
Bead size: 45–165 μm
Mean bead: size 90 μm
Maximum linear flow rate: >150 cm/h
pH stability:
Long term:3–9
Short term: 2–10
Working: 2–9
Sample: Human IgG, 2.2 mg/ml
Flow Rate: 0.3 ml/min

Protocol The column was equilibrated with binding buffer, then apply the sample at fow rate of 0.3 ml/min, when the flowthrough concentration reach 0.22 mg/min stop loading and wash the columm with biding buffer, when asorbency of 280 nm decrease to base line, elute with elution buffer.
Restrictions For Research Use only
Format Liquid
Buffer Binding Buffer: 50 mM Tris, 100 mM NaCl, pH 8.0
Elution Buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
Handling Advice Do not freeze the resin.
Storage 4 °C
Storage Comment For storage, keep the medium at 2°C to 8°C in a suitable bacteriostat, e.g. 20% ethanol and/or 0.02% sodium azide.
Product cited in: Bujak, Pretto, Ritz, Gualandi, Wulhfard, Neri: "Monoclonal antibodies to murine thrombospondin-1 and thrombospondin-2 reveal differential expression patterns in cancer and low antigen expression in normal tissues." in: Experimental cell research, Vol. 327, Issue 1, pp. 135-45, 2014 (PubMed).

Bujak, Matasci, Neri, Wulhfard: "Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification." in: Methods in molecular biology (Clifton, N.J.), Vol. 1131, pp. 315-34, 2014 (PubMed).