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Binding capacity greater than 20 mg/ml of wet gelHigh flow rateLow falling off of rProtein A/GpH stability 2-10.
Binding buffer: 0.05 M sodium borate, 0.15 M sodium chloride pH 8.0Elution buffer: 0.1 M citric acid, pH 2.75
Procedure Example:1. Wash column with ddH2O to remove air bubbles.2. Fill column with protein A/G beads.3. Wash the column with 5X volume of Binding Buffer.4. Dilute serum sample with Binding Buffer (1:1 ratio).5. Invert the diluted serum sample to mix well. Make sure no bubbles in the solution.6. Pour the solution onto the column.7. Collect the solution and repeat step 6 & 7 for 10 times.8. Wash the column 4 – 5 times with Binding Buffer containing 0.5 M NaCl9. Wash the column 4 - 5 times with the Binding Buffer.10. Add Elution Buffer to elute IgG (0.5-1 ml each time).11. Collect the eluent using microcentrifuge tube.12. Assay protein concentration and combine the fractions containing sufficient amount of IgG.13. To regenerate/store column: a. Wash with 3 volumes of elution buffer. b. Wash with 3 volumes of distilled water. c. Store column in 20 % Ethanol/H2O.