ATAD5 (Human) antibody

Overview

Antigen	ATAD5 / FRAG1
Gene ID	79915
Immunogen	Peptide with sequence C-DNKIYPKNTKKKRVD, from the internal region of the protein sequence according to NP_079133.3
Reactivity	Human
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: ATAD5 Accession Number(s): NP_079133.3. . Principal Names: ATAD5, C17orf41, chromosome 17 open reading frame 41 , FLJ12735, FRAG1, chromosome fragility associated gene 1, ATPase family, AAA domain containing 5.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human

Application Notes	Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 50kDa band in lysates of all Human cell lines tested after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 208kDa according to NP_079133.3. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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