ATGL / PNPLA2 (Human) antibody

Overview

Antigen	ATGL / PNPLA2
Gene ID	57104 , 66853 (mouse)
Immunogen	Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_080078.1
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: pnpla2 Accession Number(s): NP_080078.1. . Principal Names: pnpla2, patatin-like phospholipase domain containing 2, 0610039C21Rik, 1110001C14Rik, ATGL, TTS-2.2 , adipose triglyceride lipase, desnutrin, transport-secretion protein, triglyceride hydrolase.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Adipose, Heart and Testis lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 47.4kDa according to NP_080078.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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