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Product details for Adeno-Associated Virus 2 (VP1) antibody
Adeno-Associated Virus 2 (VP1) antibody
Overview
| Antigen: | Adeno-Associated Virus 2 (VP1) |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Host: | Mouse |
| Quantity: | |
| Price: | 400,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN112133 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Adeno-Associated Virus 2 (VP1) antibody
| Antigen | Adeno-Associated Virus 2 (VP1) |
| Immunogen | Adeno-associated virus capsid proteins and virus Particles. |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Clone | A1 |
| Host | Mouse |
| Specificity | A1 reacts with VP1 of adeno-associated virus. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al., see below) identified aa123 to aa131 of VP1 capsid protein as the specific binding region. AAV-2, found in human and monkey, weak cross-reaction with serotypes 1, 3, 4, 5 and 6. |
Application Details: Adeno-Associated Virus 2 (VP1) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Immunofluorescence microscopy. Immunoprecipitation. Immunohistochemistry on frozen and paraffin embedded sections: 1/10 - 1/20 in PBS or Tris-buffered saline. Incubation time: 3 h at room temperature or overnight at 2-8°C for immunohistochemical application. Western Blot. ELISA. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This antibody is supplied as liquid, Protein A affinity purified immunoglobulin fraction in PBS buffer with 0.09% sodium azide as preservative and 0.5% BSA as stabilizer. Reconstituted with 1 ml dist. Water. |
| Storage | Store the lyophilized antibody at 4°C. After reconstitution store the antibody at 4°C for up to one month. Store at -20°C for longer. Shelf life: one year from despatch. |
| Research Area | Virology |
| Restrictions | For Research Use only |
Publications: Adeno-Associated Virus 2 (VP1) antibody
| Publications |
Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." In: Journal of virology , Vol. 71, Issue 2, pp. 1341-52, 1997/01 (PubMed) |
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