Adeno-Associated Virus 2 antibody

Overview

Antigen:	Adeno-Associated Virus 2 (VP1/VP2)
Antibody Type:	Monoclonal
Application:	Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Host:	Mouse
Quantity:	50 ug
Price:	400,00 € (Plus shipping costs and VAT)
Order number:	ABIN112134
Availability:	3 to 10 days until shipment

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Additional Information: Adeno-Associated Virus 2 antibody

Antigen	Adeno-Associated Virus 2 (VP1/VP2)
Immunogen	Adeno-associated virus capsid proteins and virus particles.
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Clone	A69
Host	Mouse
Specificity	A69 reacts with VP 1 and VP 2 of adeno-associated virus which are highly enriched in the nucleus. Epitope mapping experiments (Wobus et al., see below) identified aa169 to aa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as the specific binding region. Reacts with AAV-2, found in human and monkey. Weak cross-reaction with serotypes 1, 3, and 6.

Application Details: Adeno-Associated Virus 2 antibody

Application	Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	Immunofluorescence microscopy. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/10. Western Blot. Incubation time: 1h at RT. Dilution buffer: PBS or Tris-buffered saline. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody was lyophilised from buffer with 0.09% sodium azide as preservative and 0.5% BSA as stabilizer. Reconstitute with 1 ml distilled water.
Storage	Store the antibody at 2-8°C after reconstitution. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Virology
Restrictions	For Research Use only

Publications: Adeno-Associated Virus 2 antibody

Publications

Wistuba, Weger, Kern et al.:
"Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins."
In: Journal of virology , Vol. 69, Issue 9, pp. 5311-9, 1995/01 (PubMed)

Wobus, Huegle-Doerr, Girod et al.:
"Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection."
In: Journal of virology , Vol. 74, Issue 19, pp. 9281-93, 2000/01 (PubMed)

External Information: Adeno-Associated Virus 2 antibody

Google Scholar	Find more references for clone A69 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Adeno-Associated Virus 2 (VP1/VP2) (Bioinformatic Harvester (EMBL))