Product details for  Adeno-Associated Virus 2 antibody
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Adeno-Associated Virus 2 antibody

Overview

Antigen: Adeno-Associated Virus 2 (VP1/VP2/VP3)
Antibody Type: Monoclonal
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Host: Mouse
Quantity: 50 ug
Price: 400,00 €   (Plus shipping costs and VAT)
Order number: ABIN112135
Availability: 3 to 10 days until shipment
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Additional Information:  Adeno-Associated Virus 2 antibody

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Antigen Adeno-Associated Virus 2 (VP1/VP2/VP3)
Immunogen Adeno-associated virus capsid proteins and virus particles.
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone B1
Host Mouse
Specificity Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. ABIN112130). B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., see below) identified aa726 to aa733 (C-terminus, common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. Reacts with AAV-2, found in human and monkey, and all published (tested so far) AAV serotypes (AAV-4 only weak cross-reaction).

Application Details:  Adeno-Associated Virus 2 antibody

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Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes Immunofluorescence microscopy. Immunoprecipitation (mainly free VP proteins). Immunohistochemistry on frozen sections: dilute reconstituted antibody 1:10 with PBS or Tris-buffered saline. Incubation time: 1h at RT. Also useful for Western Blot or Affinity chromatography. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer This Protein A affinity purified antibody was lyophilised from buffer with 0.09% sodium azide as preservative and 0.5% BSA as stabilizer. Reconstitute with 1 ml distilled water.
Storage Store the antibody at 2-8°C after reconstitution. Shelf life: one year from despatch.
Research Area Virology
Restrictions For Research Use only

Publications:  Adeno-Associated Virus 2 antibody

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Publications Wistuba, Kern, Weger et al.:
"Subcellular compartmentalization of adeno-associated virus type 2 assembly."
In: Journal of virology , Vol. 71, Issue 2, pp. 1341-52, 1997/01 (PubMed)

Wistuba, Weger, Kern et al.:
"Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins."
In: Journal of virology , Vol. 69, Issue 9, pp. 5311-9, 1995/01 (PubMed)

Wobus, Huegle-Doerr, Girod et al.:
"Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection."
In: Journal of virology , Vol. 74, Issue 19, pp. 9281-93, 2000/01 (PubMed)

External Information:  Adeno-Associated Virus 2 antibody

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Google Scholar Find more references for clone B1 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Adeno-Associated Virus 2 (VP1/VP2/VP3) (Bioinformatic Harvester (EMBL))