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Product details for Adeno-associated Virus (AAV), Intact Particles antibody
Adeno-associated Virus (AAV), Intact Particles antibody
Overview
| Antigen: | Adeno-associated Virus (AAV), Intact Particles |
| Antibody Type: | Monoclonal |
| Application: | Enzyme Immunometric Assay (EIA), Immunofluorescense (IFA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP) |
| Host: | Mouse |
| Quantity: | |
| Price: | 631,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN234198 |
| Availability: | 14-21 Business Days |
Additional Information: Adeno-associated Virus (AAV), Intact Particles antibody
| Antigen | Adeno-associated Virus (AAV), Intact Particles |
| Immunogen | Adeno-associated virus capsid proteins and virus particles |
| Antibody Type | Monoclonal |
| Format | Purified, Lyophilized |
| Isotype | IgG3 »Matching secondary antibodies |
| Description | MAb to AAV (intact) Monoclonal Antibody to Adeno-associated Virus (AAV), intact particles |
| Clone | A20 |
| Host | Mouse |
| Specificity | Reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids that is not present in denatured capsid proteins and native but unassembled capsid proteins. |
Application Details: Adeno-associated Virus (AAV), Intact Particles antibody
| Application | Enzyme Immunometric Assay (EIA), Immunofluorescense (IFA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP) |
| Application Notes | Immunofluorescence microscopy (1:20) Immunohistochemistry (frozen sections) Immunoprecipitation (1:5) Affinity chromatography ELISA Does not work in Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Reconstitution: Reconstitute with 1ml distilled water. |
| Concentration | 50ug/ml (prior to lyophilization) |
| Purification | Protein A chromatography |
| Buffer | PBS containing 0.5% BSA Preservative: 0.09% Sodium Azide |
| Storage | Store (up to 1 year after reconstitution) at 2-8°C. .Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 – 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. |
| Restrictions | For Research Use Only |
Publications: Adeno-associated Virus (AAV), Intact Particles antibody
| Publications |
Grimm, Kern, Pawlita et al.: "Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2." In: Gene therapy , Vol. 6, Issue 7, pp. 1322-30, 2000/01 (PubMed) Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." In: Journal of virology , Vol. 71, Issue 2, pp. 1341-52, 1997/01 (PubMed) Wobus, Huegle-Doerr, Girod et al.: "Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection." In: Journal of virology , Vol. 74, Issue 19, pp. 9281-93, 2000/01 (PubMed) |
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