Adeno-associated Virus VP1,VP2,VP3 antibody

Overview

Antigen:	Adeno-associated Virus VP1,VP2,VP3
Antibody Type:	Monoclonal
Application:	Immunofluorescense (IFA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
Host:	Mouse
Quantity:	50ug (Not applicable)
Price:	631,00 € (Plus shipping costs and VAT)
Order number:	ABIN234201
Availability:	7-10 Business Days

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Additional Information: Adeno-associated Virus VP1,VP2,VP3 antibody

Antigen	Adeno-associated Virus VP1,VP2,VP3
Immunogen	Adeno-associated virus capsid proteins and virus particles
Antibody Type	Monoclonal
Format	Purified, Lyophilized
Isotype	IgG1 »Matching secondary antibodies
Description	MAb to AAV VP1,VP2,VP3 Monoclonal Antibody to Adeno-associated Virus (AAV), VP1, VP2 and VP3 Tissue culture
Clone	B1
Host	Mouse
Specificity	Reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope identified a.a. 726 – a.a. 733 (C-terminus, common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. AAV-2 found in human and monkey.

Application Details: Adeno-associated Virus VP1,VP2,VP3 antibody

Application	Immunofluorescense (IFA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	Immunofluorescence microscopy Immunohistochemistry (1:10) Immunoprecipitation (mainly free VP proteins) Immunoblotting Affinity chromatography Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded..Catalog #ABIN234201 Reconstitution: Reconstitute with 1ml distilled water.
Concentration	Not applicable
Purification	Protein A chromatography
Buffer	Lyophilized from PBS containing 0.5% BSA Preservative: 0.09% Sodium azide
Storage	Store lyophilized product at 2-8°C. After reconstitution, store at 2-8°C.Warning: This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 – 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Restrictions	For Research Use Only

Publications: Adeno-associated Virus VP1,VP2,VP3 antibody

Publications

Wistuba, Weger, Kern et al.:
"Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins."
In: Journal of virology , Vol. 69, Issue 9, pp. 5311-9, 1995/01 (PubMed)

Wistuba, Kern, Weger et al.:
"Subcellular compartmentalization of adeno-associated virus type 2 assembly."
In: Journal of virology , Vol. 71, Issue 2, pp. 1341-52, 1997/01 (PubMed)

Wobus, Huegle-Doerr, Girod et al.:
"Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection."
In: Journal of virology , Vol. 74, Issue 19, pp. 9281-93, 2000/01 (PubMed)

External Information: Adeno-associated Virus VP1,VP2,VP3 antibody

Google Scholar	Find more references for clone B1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Adeno-associated Virus VP1,VP2,VP3 (Bioinformatic Harvester (EMBL))