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Product details for Adenovirus Fiber monomer and trimer antibody
Adenovirus Fiber monomer and trimer antibody
Overview
| Antigen: | Adenovirus Fiber monomer and trimer |
| Antibody Type: | Monoclonal |
| Application: | Immunofluorescence (IF), Western Blotting (WB) |
| Host: | Mouse |
| Quantity: | |
| Price: | 281,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN145806 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Adenovirus Fiber monomer and trimer antibody
| Antigen | Adenovirus Fiber monomer and trimer |
| Immunogen | UV irradiated Ad2 virus. |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C terminus that contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion. |
| Clone | 4D2 |
| Host | Mouse |
| Specificity | ABIN145806 recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kDa) only) and unboiled (both monomers and trimers (180-200kDa bands)) fibres on Western blots, and by indirect immunofluorescence studies of cells infect |
Application Details: Adenovirus Fiber monomer and trimer antibody
| Application | Immunofluorescence (IF), Western Blotting (WB) |
| Application Notes | Not tested in applications other than those listed above. This antibody is not suitable for inhibition of Ad2 attachment. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62kDa (monomer) and 180-200kDa (trimer), which corresponds to the predicted molecular weight of Adenovirus Fiber. |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
| Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| Storage | Refrigerate at 4 C. Do not freeze. |
| Restrictions | For research use only |
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