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Product details for Adenovirus Fiber trimer antibody
Adenovirus Fiber trimer antibody
Overview
| Antigen: | Adenovirus Fiber trimer |
| Antibody Type: | Monoclonal |
| Application: | Immunofluorescence (IF), Western Blotting (WB) |
| Host: | Mouse |
| Quantity: | |
| Price: | 281,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN144460 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Adenovirus Fiber trimer antibody
| Antigen | Adenovirus Fiber trimer |
| Immunogen | UV irradiated Ad2 virus. |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta sheet and beta bends, and a knob at the C terminus that contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion. |
| Clone | 2A6 |
| Host | Mouse |
| Specificity | ABIN144460 recognizes only trimers (high molecular weight bands 180-200kDa) on Western blots of unboiled Ad2 and Ad5 fiber proteins and shows immunofluorescence with Ad5 fiber mutant H5ts142 infected cells at 32??C but not at 39.5??C. |
Application Details: Adenovirus Fiber trimer antibody
| Application | Immunofluorescence (IF), Western Blotting (WB) |
| Application Notes | Immunofluorescence: assay dependent. Western Blotting: use at a concentration of 1ug / ml for 2hrs at RT. This antibody is not suitable for inhibition of Ad2 attachment. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 180-200 kDa, which corresponds to the predicted molecular weight of Adenovirus Fiber. |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
| Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| Storage | Refrigerate at 4 C. Do not freeze. |
| Restrictions | For research use only |
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