Arrestin beta 1 pSer412 (Human) antibody

Overview

Antigen:	Arrestin beta 1 pSer412
Antibody Type:	Polyclonal
Application:	Western Blotting (WB)
Reactivity:	Rat, Mouse (Murine)
Host:	Rabbit
Quantity:	0.1 ml (0.75 mg/ml)
Price:	360,00 € (Plus shipping costs and VAT)
Order number:	ABIN113648
Availability:	3 to 10 days until shipment

Hints on ordering

Add to basket

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information: Arrestin beta 1 pSer412 (Human) antibody

Antigen	Arrestin beta 1 pSer412
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat ß-arrestin-1 that contains serine 412.
Reactivity	Rat, Mouse (Murine)
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	ß-arrestin-1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, ß-arrestin-1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., ß2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. ß-arrestin-1 in the cytosol is phosphorylated by ERK1&,2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras . Raf . ERK1&,2 signaling pathway. The antibody reacts with mouse and rat ß-arrestin-1. Human and cow (77% homologous) ß-arrestin-1 have not been tested, but are expected to react.

Application Details: Arrestin beta 1 pSer412 (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot. For Western blot applications, using the antibody at 0.1-1.0 ug/mL is recommended. At 0.75 ug/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Positive controls used: PC12 and CHO-K cells. 1
Concentration	0.75 mg/ml
Buffer	Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ß-ar
Storage	Store at -80°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Research Area	Signaling, Phospho-specific antibodies
Restrictions	For Research Use only

Publications: Arrestin beta 1 pSer412 (Human) antibody

Publications

Sunose, Takeyoshi, Kawate et al.:
"[A recurrent endocrine cell cancer of the stomach showing almost complete remission after chemotherapy for 1 year]"
In: Gan to kagaku ryoho. Cancer & chemotherapy , Vol. 33, Issue 13, pp. 2073-6, 2007/01 (PubMed)

External Information: Arrestin beta 1 pSer412 (Human) antibody

Google Scholar	Find more references for antigen Arrestin beta 1 pSer412 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Arrestin beta 1 pSer412 (Bioinformatic Harvester (EMBL))