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Botulinum neurotoxin type B (BoNT/B) (Human) antibody
Overview
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| Antigen: | Botulinum neurotoxin type B (BoNT/B) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Cell-ELISA |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 250,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN179725 |
| Availability: | 1 to 2 days until shipment |
Additional Information: Botulinum neurotoxin type B (BoNT/B) (Human) antibody
| Antigen | Botulinum neurotoxin type B (BoNT/B) |
| Immunogen | Genetic immunisation with cDNA encoding human PSA |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C1,D,E,F, and G (1). BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release (2,3). BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment (4). |
| Clone | GR-3G7 |
| Host | Mouse |
Application Details: Botulinum neurotoxin type B (BoNT/B) (Human) antibody
| Application | Flow Cytometry (FACS), Cell-ELISA |
| Application Notes | FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400 |
| Concentration | 2 mg/ml |
| Purification | Protein G |
| Buffer | Phosphate buffered saline, pH 7.2 |
| Storage | Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing) |
| Restrictions | For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye |
Images: Botulinum neurotoxin type B (BoNT/B) (Human) antibody
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FACS analysis of BOSC23 cells using GR-3G7. BOSC23 cells were transiently trans-fected with an expression vector encoding eitherBoNT/B(red curve) or an irrelevant protein (controltransfectant). Binding of GR-3G7 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with BoNT/B transfected cells.
BOSC23 cells were transiently transfected withexpression vectors containing the cDNA of the lightchain of botulinum toxin A-F. Expression of the constructs was tested with an anti-myc antibody (greencurves). An irrelevant monoclonal antibody served as an
SDS-PAGE analysis of purified GR-3G7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug ofpurified GR-3G7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent. |
External Information: Botulinum neurotoxin type B (BoNT/B) (Human) antibody
| Google Scholar | Find more references for clone GR-3G7 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Botulinum neurotoxin type B (BoNT/B) (Bioinformatic Harvester (EMBL)) |
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