Cortactin (phospho Y466) (Human) antibody

Overview

Antigen	Cortactin (phospho Y466)
Immunogen	Synthetic peptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence).
Reactivity	Human, Mouse (Murine)
Antibody Type	Polyclonal
Format	Liquid
Isotype	IgG »Matching secondary antibodies
Description	Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3(SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an ?? helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are Src phosphorylation sites at tyrosines 421, 466 and 482 (421, 470 and 486 in the human sequence) that when phosphorylated, attenuate the cross-linking of F-actin into meshworks.
Host	Rabbit

Application	Western Blotting (WB)
Application Notes	WB: Use at a concentration of 0.1-1.0 ug/ml. Predicted molecular weight: 83 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Concentration	0.35 mg/ml
Purity	Immunogen affinity purified
Buffer	Preservative: 0.05% Sodium AzideConstituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4
Restrictions	For research use only

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