Cortactin pTyr421 (Mouse (Murine)) antibody

Overview


	Antigen:	Cortactin pTyr421
	Antibody Type:	Polyclonal
	Application:	Immunofluorescence (IF), Western Blotting (WB)
	Reactivity:	Mouse (Murine)
	Host:	Rabbit
	Quantity:	0.1 ml (0.35 mg/ml)
	Price:	380,00 € (Plus shipping costs and VAT)
	Order number:	ABIN113675
	Availability:	3 to 10 days until shipment

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Additional Information: Cortactin pTyr421 (Mouse (Murine)) antibody

Antigen	Cortactin pTyr421
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 421.
Reactivity	Mouse (Murine)
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks. Mouse cortactin. Does not cross-react with human.

Application Details: Cortactin pTyr421 (Mouse (Murine)) antibody

Application	Immunofluorescence (IF), Western Blotting (WB)
Application Notes	The antibody has been used for Western blot applications, and immunocytochemistry. For Western blot applications, we recommend using the antibody at 0.1-1.0 µg/mL. At 0.35 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. Positive control used: 5HD47, a C3H 10T1/2 cell line overexpressing Src and transfected with mouse Cortactin. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. 1
Concentration	0.35 mg/ml
Buffer	This antibody is supplied as liquid, purified IgG in in phosphate buffer, pH 7.4, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide as preservative. Purified from rabbit serum by sequential epitope-specific chromatography. The an
Storage	Store at 4°C short term only. Aliquot and store at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	For Research Use only

Publications: Cortactin pTyr421 (Mouse (Murine)) antibody

Publications

Concejero, Chen, Wang et al.:
"Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan."
In: Transplantation , Vol. 85, Issue 3, pp. 398-406, 2008/01 (PubMed)

Images: Cortactin pTyr421 (Mouse (Murine)) antibody

Image for Cortactin pTyr421 (Mouse (Murine)) antibody (1)

External Information: Cortactin pTyr421 (Mouse (Murine)) antibody

Google Scholar	Find more references for antigen Cortactin pTyr421 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cortactin pTyr421 (Bioinformatic Harvester (EMBL))