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Product details for Cortactin pTyr466 (Mouse (Murine)) antibody
Cortactin pTyr466 (Mouse (Murine)) antibody
Overview
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| Antigen: | Cortactin pTyr466 |
| Antibody Type: | Polyclonal |
| Application: | Immunofluorescence (IF), Western Blotting (WB) |
| Reactivity: | Mouse (Murine) |
| Host: | Rabbit |
| Quantity: | |
| Price: | 380,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN113676 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Cortactin pTyr466 (Mouse (Murine)) antibody
| Antigen | Cortactin pTyr466 |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence). |
| Reactivity | Mouse (Murine) |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Host | Rabbit |
| Specificity | Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are Src phosphorylation sites at tyrosines 421, 466 and 482 (421, 470 and 486 in the human sequence) that when phosphorylated, attenuate the cross-linking of F-actin into meshworks. Mouse cortactin. Human (62% homologous) cortactin has not been tested. |
Application Details: Cortactin pTyr466 (Mouse (Murine)) antibody
| Application | Immunofluorescence (IF), Western Blotting (WB) |
| Application Notes | The antibody has been used for Western blot applications. For Western blot applications, we recommend using the antibody at 0.1-1.0 µg/mL. At 0.35 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. Positive control used: NIH3T3 cells expressing activated c-Src. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 0.35 mg/ml |
| Buffer | This antibody is supplied as liquid, purified IgG in in phosphate buffer, pH 7.4, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide as preservative. Purified from rabbit serum by sequential epitope-specific chromatography. The an |
| Storage | Store at 4°C short term only. Aliquot and store at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Restrictions | For Research Use only |
External Information: Cortactin pTyr466 (Mouse (Murine)) antibody
| Google Scholar | Find more references for antigen Cortactin pTyr466 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Cortactin pTyr466 (Bioinformatic Harvester (EMBL)) |
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