Product details for  D1-Nt (PsbA) (Arabidopsis) antibody
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D1-Nt (PsbA) (Arabidopsis) antibody

Overview

Image for D1-Nt (PsbA) (Arabidopsis) antibody
Antigen: D1-Nt (PsbA)
Antibody Type: Polyclonal
Application: Western Blotting (WB)
Reactivity: Arabidopsis, Tobacco, Cyanobacteria
Host: Rabbit
Quantity: 200 µl
Price: 250,00 €   (Plus shipping costs and VAT)
Order number: ABIN165361
Availability: 1 day until shipment

Additional Information:  D1-Nt (PsbA) (Arabidopsis) antibody

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Antigen D1-Nt (PsbA)
Immunogen Synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.
Reactivity Arabidopsis, Tobacco, Cyanobacteria
Antibody Type Polyclonal
Isotype IgG »Matching secondary antibodies
Description D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).
Host Rabbit
Specificity Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.

Application Details:  D1-Nt (PsbA) (Arabidopsis) antibody

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Application Western Blotting (WB)
Application Notes Western blot (1 : 2.000 for ECL)
Storage Short term +4°C, long term -20°. Repeated freezing and thawing is not recommended.
Research Area Plant/Algal Physiology
Restrictions For Research Use only

Images:  D1-Nt (PsbA) (Arabidopsis) antibody

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Western blot analysis of chloroplast (Arabidopsis and tobacco) and Synechocystis thylakoid proteins with anti-PsbA-Nt

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External Information:  D1-Nt (PsbA) (Arabidopsis) antibody

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