D4-GDI (Human) antibody

Overview


	Antigen:	D4-GDI (GDP dissociation inhibitor) (cleaved form)
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine)
	Host:	Mouse
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	310,00 € (Plus shipping costs and VAT)
	Order number:	ABIN121226
	Availability:	Ships in 3 to 10 days

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Additional Information: D4-GDI (Human) antibody

Antigen	D4-GDI (GDP dissociation inhibitor) (cleaved form)
Immunogen	Synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI
Reactivity	Human, Mouse (Murine)
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Clone	97A1015
Host	Mouse
Specificity	This antibody recognizes D4-GDI from human and mouse. D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Application Details: D4-GDI (Human) antibody

Application	Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	Western blotting (2 µg/ml, In anti-Fas treated Jurkat cells, a ~23 kDa band should be observed). Flow Cytometry (intracellular, 0.5 – 1 µg for 106 cells). Immunoprecipitation (1-2 µg/ml). Immunohisto-chemistry (2 µg/ml). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid, Protein G purified IgG fraction in PBS with 0.02% sodium azide as preservative. Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were
Storage	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and tha wing. Shelf life: one year from despatch.
Restrictions	Research only

Images: D4-GDI (Human) antibody

Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI cleavage was detected by ABIN121226.

Image for D4-GDI (Human) antibody (1)

External Information: D4-GDI (Human) antibody

Google Scholar	Find more references for clone 97A1015 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen D4-GDI (GDP dissociation inhibitor) (cleaved form) (Bioinformatic Harvester (EMBL))