Product details for  DARPP-32 pSer75 (Human) antibody
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DARPP-32 pSer75 (Human) antibody

Overview

Antigen: DARPP-32 pSer75
Antibody Type: Polyclonal
Application: Western Blotting (WB), Immunoblotting (IB)
Reactivity: Human, Mouse (Murine)
Host: Rabbit
Quantity: 0.1 ml
Price: 380,00 €   (Plus shipping costs and VAT)
Order number: ABIN152574
Availability: Ships in 7 to 10 days
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Additional Information:  DARPP-32 pSer75 (Human) antibody

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Antigen DARPP-32 pSer75
Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding thephospho Thr75 of DARPP-32. The sequence of the immunogen is identical in humanand mouse.
Reactivity Human, Mouse (Murine)
Antibody Type Polyclonal
Format Aff - Purified
Description DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac®.
Host Rabbit
Specificity Specific for the ~34k DARPP-32 phosphorylated at Thr75. This antibody recognizes human, mouse, and rat forms of the protein. Other specieshave not been tested.

Application Details:  DARPP-32 pSer75 (Human) antibody

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Application Western Blotting (WB), Immunoblotting (IB)
Application Notes Western and Dot blots. Suggested working dilutions: *Immunoblot - 1:1,000Immunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application.
Format Aff - Purified
Buffer 0.1 ml affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mlBSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blot.
Storage For long term storage –80 degrees Celcius is recommended, but shorter term storage at–20 degrees Celcius is also acceptable as aliquots may be taken without freeze/thawingdue to the presence of 50% glycerol.
Restrictions For Research Use only

External Information:  DARPP-32 pSer75 (Human) antibody

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Google Scholar Find more references for antigen DARPP-32 pSer75 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen DARPP-32 pSer75 (Bioinformatic Harvester (EMBL))