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Product details for DEFCAP (Human) antibody
DEFCAP (Human) antibody
Overview
| Antigen: | DEFCAP |
| Antibody Type: | Polyclonal |
| Application: | ELISA, Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 242,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN147908 |
| Availability: | 7 to 10 days until shipment |
Additional Information: DEFCAP (Human) antibody
| Antigen | DEFCAP |
| Immunogen | Synthetic peptide WEKGSKKGLLPLSScorresponding to C-term of human DEFCAP in both theDEFCAP-L and ?S isoforms. |
| Reactivity | Human, Rat |
| Antibody Type | Polyclonal |
| Format | Liquid |
| Isotype | IgG »Matching secondary antibodies |
| Description | Death Effector Filament-forming Ced 4 like Apoptosis Protein (DEFCAP) is a novel apoptotic protein of the mammalian Ced 4 family. It has two isoforms: DEFCAP-Land DEFCAP-S. The deduced amino acid sequences of these two alternately spliced isoforms only differ by 44amino acids at the C-terminus. DEFCAP contains a caspase recruitment domain (CARD), a domain that with the proper stimulation can recruit caspases to form cytoplasmic structures called death effector filaments, which in turn initiate the apoptotic signaling pathway. |
| Host | Rabbit |
| Specificity | This antibody reacts predominantly with DEFCAP. |
Application Details: DEFCAP (Human) antibody
| Application | ELISA, Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Immunoprecipitation: use at a concentration of 3.0-5.0 ug / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5-2.0 ug/ml. ELISA: use at a concentration of 0.1-1.0 ug/ml. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 165 kDa in rat brain tissue lysate, which corresponds to the predicted molecular weight of DEFCAP. Sample wasboiled prior to subjecting on 7.5% SDS-PAGE. An ~80 kDa band was also observed in this blot. |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Buffer | PBS with 0.02% sodium azide |
| Storage | Store at 2-8 C for up to one month. Aliquot and store at -20 C long term. Avoid repeated freeze / thaw cycles. |
| Restrictions | For research use only |
External Information: DEFCAP (Human) antibody
| Google Scholar | Find more references for antigen DEFCAP on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen DEFCAP (Bioinformatic Harvester (EMBL)) |
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