DICE-1 (Human) antibody

Overview

Antigen	DICE1/DDX26
Immunogen	A synthetic peptide which maps to a region between residues 225 and 275 of humanDICE1/DDX26, DEAD/H box polypeptide using the numbering given in TrEMBL entryQ9UL03 (GeneID 26512).
Reactivity	Human, Mouse (Murine)
Antibody Type	Polyclonal
Format	Aff - Purified
Description	The tumor suppressor gene DICE1 is located within a previously reported critical regionof loss of heterozygosity on chromosome 13q14.3. Expression of the remaining DICE1allele is down-regulated in non-small cell lung carcinomas. In IGF-IR transformed Balb/c3T3, DICE1 substantially suppressed growth in soft agar. These results demonstratethat DICE1 has a growth-suppressing activity and interferes withanchorage-independent growth of IGF-IR transformed tumor cells dependent upon IGF-Isignaling. Synonyms: DICE1 antibody, deleted in cancer 1 antibody, DDX26 antibody, DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 26 antibody, HDB antibody, RNA helicaseHDB/DICE1 antibody
Host	Rabbit
Specificity	NB 200-180 is specific for human DICE1/DDX260 protein. Human. Other species not tested. Reactivity with mouse DICE1/DDX26 is expectedowing to conservation of sequence between this ortholog and human DICE1/DDX26 atthe site to which the epitope maps.

Application	Western Blotting (WB)
Application Notes	Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 - 1:10,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested*In some cases, the antibody may be diluted further than indicated. Positive Control:HeLa nuclear extract (NB 800-PC9)
Concentration	1 mg/ml
Buffer	0.1 ml of affinity purified rabbit sera in a Tris-citrate/phosphate (pH 7-8) buffer with 0.1%sodium azide.
Storage	Store at 4 degrees Celcius.
Restrictions	For Research Use only

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