E2-EPF (Human) antibody

Overview

Antigen	E2-EPF
Gene ID	27338 , 77891 (mouse)
Immunogen	Peptide with sequence NSNVENLPPHIIR-C, from the N Terminus of the protein sequence according to NP_055316
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: UBE2S Accession Number(s): NP_055316 Gene Ontology terms: ubiquitin conjugating enzyme activity, ubiquitin-protein ligase activity, ubiquitin cycle, protein modification, ligase activity. . Principal Names: E2-EPF, ubiquitin carrier protein, ubiquitin-protein ligase, ubiquitin-conjugating enzyme E2-24 kD, UBE2S, ubiquitin-conjugating enzyme E2S.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx. 35-40kDa band observed in Human Liver lysate at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 25kDa according to NP_055316. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Format	Aff - Purified
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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EMBL Harvester	Search human, mouse or rat genome for antigen E2-EPF (Bioinformatic Harvester (EMBL))