ER alpha non pSer167 (Human) antibody

Overview


	Antigen:	Estrogen Receptor alpha non pSer167
	Antibody Type:	Polyclonal
	Application:	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Human, Monkey
	Host:	Rabbit
	Quantity:	50 (0.5 mg/ml)
	Price:	200,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123081
	Availability:	Ships in 3 to 10 days

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Additional Information: ER alpha non pSer167 (Human) antibody

Antigen	Estrogen Receptor alpha non pSer167
Immunogen	Synthetic peptide (GGRERLASTNDK) corresponding to amino acids 161-172 of human ER to pair the phosphorylated site of Serine 167
Reactivity	Human, Monkey
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	The estrogen receptor a (ERa), a transcription factor that controls the expression of a number of genes involved in cellular differentiation and proliferation in a wide variety of tissues (1, 2), is regulated by ligand binding and phosphorylation. ER contains at least two transcriptionally active domains: constitutively active F-1 at the ER N-terminus of the protein and ligand-dependent AF-2 at the ER C-terminus (3, 4). Although ligand binding is considered essential for the full activation of ER, it has long been recognized that the receptor is subject to post-translational alterations, such as phosphorylation, which also regulated its activity (5) ER phosphorylation patterns appear to be cell type-specific. Serine residues are the predominantly modified amino acids present in ER, and four of these (Ser-104, 106, 118, 167) are clustered in the N-terminus within AF-1 of the receptor (6). Serine 167 has been shown to be phosphorylated by p90rsk1 in vitro and to regulate ER AF-1-dependent transcriptional activation in vivo (7). ER from mouse and human become hyperphosphorlated on serine residues in the presence of estradiol. This antibody reacts with ER alpha (Paired167).

Application Details: ER alpha non pSer167 (Human) antibody

Application	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	ELISA (0.1 – 1.0 µg/ml), Western blot (0.5 – 2 µg/ml) and Immunoprecipitation (3.0 - 5.0 µg/extract from 107 cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid epitope affinity purified immunglobulin fraction in Phosphate buffered saline pH 7.4 with 0.02% sodium azide as preservative.
Storage	Store the antibody undiluted at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Research Area	Hormones, Receptors
Restrictions	Research only

Publications: ER alpha non pSer167 (Human) antibody

Publications

Blobel, Orkin:
"Estrogen-induced apoptosis by inhibition of the erythroid transcription factor GATA-1."
In: Molecular and cellular biology , Vol. 16, Issue 4, pp. 1687-94, 1996/01 (PubMed)

Ali, Metzger, Bornert et al.:
"Modulation of transcriptional activation by ligand-dependent phosphorylation of the human oestrogen receptor A/B region."
In: The EMBO journal , Vol. 12, Issue 3, pp. 1153-60, 1993/01 (PubMed)

Joel, Smith, Sturgill et al.:
"pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167."
In: Molecular and cellular biology , Vol. 18, Issue 4, pp. 1978-84, 1998/01 (PubMed)

McInerney, Tsai, OMalley et al.:
"Analysis of estrogen receptor transcriptional enhancement by a nuclear hormone receptor coactivator."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 93, Issue 19, pp. 10069-73, 1996/01 (PubMed)

Gaub, Bellard, Scheuer et al.:
"Activation of the ovalbumin gene by the estrogen receptor involves the fos-jun complex."
In: Cell , Vol. 63, Issue 6, pp. 1267-76, 1991/01 (PubMed)

Kraus, McInerney, Katzenellenbogen:
"Ligand-dependent, transcriptionally productive association of the amino- and carboxyl-terminal regions of a steroid hormone nuclear receptor."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 92, Issue 26, pp. 12314-8, 1996/01 (PubMed)

Images: ER alpha non pSer167 (Human) antibody

WB: Whole cell lysate derived from MCF-7 were probed by anti-ER-alpha (Paired 167) (Lane 1) or by pre-absorpted antibody with immunizing peptide (lane 2).

Image for ER alpha non pSer167 (Human) antibody (1)

External Information: ER alpha non pSer167 (Human) antibody

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