ER alpha pSer167 (Human) antibody

Overview


	Antigen:	Estrogen Receptor alpha pSer167
	Antibody Type:	Polyclonal
	Application:	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	50 (0.5 mg/ml)
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123080
	Availability:	Ships in 3 to 10 days

Hints on ordering

Add to basket

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information: ER alpha pSer167 (Human) antibody

Antigen	Estrogen Receptor alpha pSer167
Immunogen	Synthetic peptide corresponding to human ER at the phosphorylated site of Serine 167
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	The estrogen receptor a (ERa), a transcription factor that controls the expression of a number of genes involved in cellular differentiation and proliferation in a wide variety of tissues (1, 2), is regulated by ligand binding and phosphorylation. ER contains at least two transcriptionally active domains: constitutively active F-1 at the ER N-terminus of the protein and ligand-dependent AF-2 at the ER C-terminus (3, 4). Although ligand binding is considered essential for the full activation of ER, it has long been recognized that the receptor is subject to post-translational alterations, such as phosphorylation, which also regulated its activity (5) ER phosphorylation patterns appear to be cell type-specific. Serine residues are the predominantly modified amino acids present in ER, and four of these (Ser-104, 106, 118, 167) are clustered in the N-terminus within AF-1 of the receptor (6). Serine 167 has been shown to be phosphorylated by p90rsk1 in vitro and to regulate ER AF-1-dependent transcriptional activation in vivo (7). ER from mouse and human become hyperphosphorlated on serine residues in the presence of estradiol. This antibody reacts wit human ER alpha.

Application Details: ER alpha pSer167 (Human) antibody

Application	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	ELISA (0.1 – 1.0 µg/ml), Western blotting (0.5 – 2 µg/ml, detects a band of ~60 kDa) and Immunoprecipitation (3.0 - 5.0 µg/extract from 107 cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid epitope affinity purified immunglobulin fraction in Phosphate buffered saline pH 7.4 with 0sodium azide as preservative. .02%
Storage	Store the antibody undiluted at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Research Area	Hormones, Receptors, Phospho-specific antibodies
Restrictions	Research only

Publications: ER alpha pSer167 (Human) antibody

Publications

Blobel, Orkin:
"Estrogen-induced apoptosis by inhibition of the erythroid transcription factor GATA-1."
In: Molecular and cellular biology , Vol. 16, Issue 4, pp. 1687-94, 1996/01 (PubMed)

Ali, Metzger, Bornert et al.:
"Modulation of transcriptional activation by ligand-dependent phosphorylation of the human oestrogen receptor A/B region."
In: The EMBO journal , Vol. 12, Issue 3, pp. 1153-60, 1993/01 (PubMed)

Joel, Smith, Sturgill et al.:
"pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167."
In: Molecular and cellular biology , Vol. 18, Issue 4, pp. 1978-84, 1998/01 (PubMed)

McInerney, Tsai, OMalley et al.:
"Analysis of estrogen receptor transcriptional enhancement by a nuclear hormone receptor coactivator."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 93, Issue 19, pp. 10069-73, 1996/01 (PubMed)

Gaub, Bellard, Scheuer et al.:
"Activation of the ovalbumin gene by the estrogen receptor involves the fos-jun complex."
In: Cell , Vol. 63, Issue 6, pp. 1267-76, 1991/01 (PubMed)

Kraus, McInerney, Katzenellenbogen:
"Ligand-dependent, transcriptionally productive association of the amino- and carboxyl-terminal regions of a steroid hormone nuclear receptor."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 92, Issue 26, pp. 12314-8, 1996/01 (PubMed)

Images: ER alpha pSer167 (Human) antibody

WB: Whole cell lysate from stimulated MCF-7 were probed by anti-ER-alpha (pSer167) (Lane 1) or by pre-absorpted antibody with immunizing peptide (lane 2).

Image for ER alpha pSer167 (Human) antibody (1)

External Information: ER alpha pSer167 (Human) antibody

Google Scholar	Find more references for antigen Estrogen Receptor alpha pSer167 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Estrogen Receptor alpha pSer167 (Bioinformatic Harvester (EMBL))