ER beta (Human) antibody

Overview

Antigen:	Estrogen Receptor beta (109-122)
Antibody Type:	Polyclonal
Application:	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
Reactivity:	Rat, Mouse (Murine), Human, Monkey
Host:	Rabbit
Quantity:	50 (0.5 mg/ml)
Price:	200,00 € (Plus shipping costs and VAT)
Order number:	ABIN123082
Availability:	Ships in 3 to 10 days

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Additional Information: ER beta (Human) antibody

Antigen	Estrogen Receptor beta (109-122)
Immunogen	Synthetic peptide corresponding to amino acids 109-122 of human ERß. This sequence is identical to the mouse, rat. Only one amino acid is different from bovine.
Reactivity	Rat, Mouse (Murine), Human, Monkey
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	Estrogen regulates various physiological functions upon binding to its specific receptors. Estrogen receptors (ERs) are members of the nuclear receptor super family of ligand-dependent transcription factors that regulate the expression of target gene (1). ERa and ERß have been identified that are encoded by different genes. ERa and ERß have similar overall structures with at least three functional domains in common: The ligand-binding domain located at the C-terminal half of the proteins, the DNA-binding domain located centrally and the N-terminus (2, 3). Both ER subtypes are co-expressed in the same cells such as brain, pituitary, and mammary gland (4-9). The co-expression of the two isoforms would be a key determinant in the cellular responses to estrogen. This antibody reacts with ER beta.

Application Details: ER beta (Human) antibody

Application	Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	ELISA (0.1 – 1.0 µg/ml), Western blot (0.5 – 2 µg/ml, detects a band of ~55 kDa) and Immunoprecipitation (3.0 – 5.0 µg/extract from 107 cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid epitope affinity purified immunglobulin fraction in Phosphate buffered saline pH 7.4 with 0.02% proclin 300 as preservative.
Storage	Store the antibody undiluted at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Research Area	Hormones, Receptors
Restrictions	Research only

Publications: ER beta (Human) antibody

Publications

Matsuda, Ochiai, Nishi et al.:
"Colocalization and ligand-dependent discrete distribution of the estrogen receptor (ER)alpha and ERbeta."
In: Molecular endocrinology (Baltimore, Md.) , Vol. 16, Issue 10, pp. 2215-30, 2002/01 (PubMed)

Jefferson, Couse, Padilla-Banks et al.:
"Neonatal exposure to genistein induces estrogen receptor (ER)alpha expression and multioocyte follicles in the maturing mouse ovary: evidence for ERbeta-mediated and nonestrogenic actions."
In: Biology of reproduction , Vol. 67, Issue 4, pp. 1285-96, 2002/01 (PubMed)

Kawata:
"Roles of steroid hormones and their receptors in structural organization in the nervous system."
In: Neuroscience research , Vol. 24, Issue 1, pp. 1-46, 1996/01 (PubMed)

External Information: ER beta (Human) antibody

Google Scholar	Find more references for antigen Estrogen Receptor beta (109-122) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Estrogen Receptor beta (109-122) (Bioinformatic Harvester (EMBL))