F4 / 80 (Mouse) antibody

Overview

Antigen:	F4 / 80
Antibody Type:	Monoclonal
Application:	Flow Cytometry (FACS), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
Reactivity:	Mouse (Murine)
Host:	Rat
Quantity:	250 µg (1 mg/ml)
Price:	330,00 € (Plus shipping costs and VAT)
Order number:	ABIN145735
Availability:	7 to 10 days until shipment

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Additional Information: F4 / 80 (Mouse) antibody

Antigen	F4 / 80
Immunogen	Thioglycollate stimulated peritoneal macrophages from C57/BL mice
Reactivity	Mouse (Murine)
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG2b »Matching secondary antibodies
Description	The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells.
Clone	CI:A3-1
Host	Rat
Specificity	This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.

Application Details: F4 / 80 (Mouse) antibody

Application	Flow Cytometry (FACS), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
Application Notes	Flow Cytometry (Use 10ul of the suggested working dilution to label 10^6 cells)Immunohistology (frozen, paraffin and resin)-requires protein digestion pre-treatment of paraffin sections e.g. trypsinImmunoprecipitationWestern BlottingRadioimmunoassay
Concentration	1 mg/ml
Purity	IgG fraction
Buffer	0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Storage	Store at 4 C. Aliquot and store at -20 C long-term.
Restrictions	For research use only

Publications: F4 / 80 (Mouse) antibody

Publications

Lee, Starkey, Gordon:
"Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80."
In: The Journal of experimental medicine , Vol. 161, Issue 3, pp. 475-89, 1985/01 (PubMed)

Austyn, Gordon:
"F4/80, a monoclonal antibody directed specifically against the mouse macrophage."
In: European journal of immunology , Vol. 11, Issue 10, pp. 805-15, 1982/01 (PubMed)

Alves-Rosa, Vermeulen, Cabrera et al.:
"Macrophage depletion following liposomal-encapsulated clodronate (LIP-CLOD) injection enhances megakaryocytopoietic and thrombopoietic activities in mice."
In: British journal of haematology , Vol. 121, Issue 1, pp. 130-8, 2003/01 (PubMed)

Beck, Voswinckel, Wagner et al.:
"Participation of bone marrow-derived cells in long-term repair processes after experimental stroke."
In: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism , Vol. 23, Issue 6, pp. 709-17, 2003/01 (PubMed)

External Information: F4 / 80 (Mouse) antibody

Google Scholar	Find more references for clone CI:A3-1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen F4 / 80 (Bioinformatic Harvester (EMBL))