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Application
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Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
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Application Notes
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ELISA: It is recommended that a range of dilutions be utilized in prelimary experiments to determine optimal concentrations for use of this antibody. For most applications, the anti-human VEGFR-2 sc-Fv antibody should be diluted between 1:50 and 1:20 fold. FACS: To detect surface expressed VEGFR-2 on HUVECs and CD34+VEGFR-2+ hematopoetic immature cells by FACS analysis this antibody can be used at concentrations of 0.5-5 ug/ml. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Detection Note: The VEGFR-2 sc-Fv antibody has an E-tag for detection by secondary antibodies (e.g. anti-E-tag mouse monoclonal antibody, Amersham Pharmacia No. 27-9412-01).
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Concentration
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0.2 mg/ml Source: Anti-human VEGFR-2 single chain (VEGFR-2 sc-Fv) antibody was constructed from cDNA of immunised mice and produced in E.coli.
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Buffer
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This antibody is supplied as liquid, affinity purified single chain antibody in PBS (Sodium chloride 150 mM, Sodium phosphate 150 mM pH 7.2) without preservatives or stabilizers.
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Research Area
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Cytokines, Receptors
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Restrictions
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For Research Use only
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