FABP4 (Human) antibody

Overview

Antigen:	Fatty Acid Binding Protein 4 (FABP4, A-FABP)
Antibody Type:	Polyclonal
Application:	Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Reactivity:	Human
Host:	Rabbit
Quantity:	0.05 mg
Price:	448,63 $ (Plus shipping costs and VAT, Invoice in EUR)
Order number:	ABIN115503
Availability:	3 to 10 days until shipment

Hints on ordering

Add to basket

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information: FABP4 (Human) antibody

Antigen	Fatty Acid Binding Protein 4 (FABP4, A-FABP)
Immunogen	Recombinant FABP4 from E. coli
Reactivity	Human
Antibody Type	Polyclonal
Format	Purified
Host	Rabbit
Specificity	Adipocyte fatty acid binding protein FABP4 is a 15 kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. FABP4 is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells. In mice, targeted mutations in FABP4 provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from FABP4deficient mice also have reduced efficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated FABP4 expression in macrophages upon differentiation and activation. In these cells, FABP4 modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific FABP4 deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for FABP4 in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages. The recombinant human FABP4 is 100% homologous with the human serum FABP4.

Application Details: FABP4 (Human) antibody

Application	Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Application Notes	Western blot. ELISA (Titre is defined by indirect ELISA, it is: >1:100,000 for antibody concentration 1mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody is supplied as immunoglobulin fraction, affinity purified with immobilized human FABP4, sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. Add 0.05 ml of deionized water and let the lyophilized pell
Storage	Store lyophilized antibody at 2-8°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Shelf life: 1 year from despatch.
Research Area	Enzymes, Metabolism
Restrictions	For Research Use only

Publications: FABP4 (Human) antibody

Publications

Prinsen, de Bruijn, Merkx et al.:
"Assignment of the human adipocyte fatty acid-binding protein gene (FABP4) to chromosome 8q21 using somatic cell hybrid and fluorescence in situ hybridization techniques."
In: Genomics , Vol. 40, Issue 1, pp. 207-9, 1997/01 (PubMed)

External Information: FABP4 (Human) antibody

Google Scholar	Find more references for antigen Fatty Acid Binding Protein 4 (FABP4, A-FABP) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Fatty Acid Binding Protein 4 (FABP4, A-FABP) (Bioinformatic Harvester (EMBL))