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Product details for BIRC3 (Human) antibody
BIRC3 (Human) antibody
Overview
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| Antigen: | IAP1 (BIRC3) |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB), Functional Studies (FUNC) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Goat |
| Quantity: | |
| Price: | 300,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN184600 |
| Availability: | 3 to 10 days until shipment |
Additional Information: BIRC3 (Human) antibody
| Antigen | IAP1 (BIRC3) |
| Gene ID | 330 , 11797 (mouse) |
| Immunogen | Peptide with sequence C-RSTIKGTVRT, from the C Terminus of the protein sequence according to NP_001156.1, NP_001156.1 |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Description | Official Gene Symbol: BIRC3 Accession Number(s): NP_001156.1, NP_001156.1 Gene Ontology terms: apoptosis inhibitor activity, anti-apoptosis, cell surface receptor linked signal transduction, intracellular. . Principal Names: BIRC3, AIP1, API2, MIHC, CIAP2, HAIP1, HIAP1, RNF49, cIAP2, hiap-1, MALT2, baculoviral IAP repeat-containing 3, apoptosis inhibitor 2, TNFR2-TRAF signalling complex protein, inhibitor of apoptosis protein 1, mammalian IAP homolog. |
| Host | Goat |
| Specificity | Expected reactivity from sequence similarity: Human, Mouse, Rat |
Application Details: BIRC3 (Human) antibody
| Application | Western Blotting (WB), Functional Studies (FUNC) |
| Application Notes | Western blot: Approx 80 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/ml. Please note that we get a slightly higher MW band compared to the predicted size of 66kDa according to NP_001156 . We did not find anything in the literature to suggest otherwise. Peptide neutralisation experiments indicate that the 80kDa band is completely blocked when primary antibody is incubated with the blocking peptide. Moreover, BIRC3 has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use Only |
External Information: BIRC3 (Human) antibody
| Google Scholar | Find more references for antigen IAP1 (BIRC3) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen IAP1 (BIRC3) (Bioinformatic Harvester (EMBL)) |
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