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Product details for Indolacetic Acid antibody
Indolacetic Acid antibody
Overview
| Antigen: | Indolacetic Acid |
| Antibody Type: | Polyclonal |
| Host: | Rabbit |
| Quantity: | |
| Price: | 471,50 € (Plus shipping costs and VAT) |
| Order number: | ABIN165489 |
| Availability: | 7 days until shipment |
Additional Information: Indolacetic Acid antibody
| Antigen | Indolacetic Acid |
| Immunogen | Synthetic Indole 3 Acetic Acid conjugated to protein carriers. |
| Antibody Type | Polyclonal |
| Format | Lyophilized |
| Isotype | IgG »Matching secondary antibodies |
| Host | Rabbit |
| Specificity | Conjugated Indole 3 acetic Acid. . Using a conjugate Indole 3 Acetic acid-protein carrier, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) Indole 3 Acetic Acid-BSA 1 Indole 3 Pyruvic Acid-BSA 1/>50,000 Phenyl Acetic Acid-BSA 1/>50,000 (a) |
Application Details: Indolacetic Acid antibody
| Application Notes | Example of immunohistochemistry application Tissues fixation:1-Samples are fixed with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in a 4-Morpholino ethanesulfonic acid (MES 10-1M pH 6.3) buffer. 2-After dehydration with a graded series of ethanol, get the wax section. Example of immunohistochemistry protocol:1-In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2-Then, wash the sections for 20 min in 0.15 M phosphate-buffered saline (PBS) (pH 7.2) 3-Pre-incubate for 30 min in PBS containing 10% of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4-Incubate at room temperature (1h 30min) and overnight at 4º C in the same mixed solution containing anti- conjuguated Indole-3-acetic acid antibodies(diluted 1/1,000 to 1/5,000 as recommended dilution). 5-Then, the sections will be wash in PBS (30 min). 6-After that we will incubate for 60 min at room temperature with biotinylated anti-rabbit immunogammaglobulin (Vector) diluted 1/200 in PBS. 7-Wash during 30 min with PBS. 8-Sections will be incubated for 1 h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain). 9-After that we will wash the sections in PBS (30 min) 10-Wash with Tris-HCl buffer (pH 7.6)(10 min). 11-The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3' diaminobenzidine as chromogen. 12-Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). |
| Purity | Antiserum previously absorbed on protein carriers, and purified |
| Storage | After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times), and stable at least 2 years. |
| Restrictions | For Research Use only |
External Information: Indolacetic Acid antibody
| Google Scholar | Find more references for antigen Indolacetic Acid on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Indolacetic Acid (Bioinformatic Harvester (EMBL)) |
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