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Product details for Interleukin-1 beta (IL-1-beta) (Mouse) antibody
Interleukin-1 beta (IL-1-beta) (Mouse) antibody
Overview
| Antigen: | Interleukin-1 beta (IL-1-beta) |
| Antibody Type: | Polyclonal |
| Application: | Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Mouse (Murine) |
| Host: | Rabbit |
| Quantity: | |
| Price: | 287,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN103019 |
| Availability: | 7 days until shipment |
Additional Information: Interleukin-1 beta (IL-1-beta) (Mouse) antibody
| Antigen | Interleukin-1 beta (IL-1-beta) |
| Immunogen | . |
| Reactivity | Mouse (Murine) |
| Antibody Type | Polyclonal |
| Format | Liquid (sterile filtered) |
| Host | Rabbit |
Application Details: Interleukin-1 beta (IL-1-beta) (Mouse) antibody
| Application | Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Purity | NoneStabilizer: None Preservative: None |
| Purification | This antiserum has been heated to 56° C for 30 minutes. This antibody is primarily directed against mature, 17,000 MW mouse IL-1 and is useful in determining its presence in various assays. The antiserum does not recognize human IL-1 or mouse IL-1. In ELISA formats and other immunoreactive assays, reactivity with rat IL-1 is variable and depends on the capture antibody. This antiserum will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 precursor molecule. Denatured 31,000 precursor IL-1 will be recognized by this antibody. This is due to the unfolding of the denatured precursor IL-1 exposing epitopes not exposed in the natural state. This antibody is also useful for neutralization of mouse IL-1 activity in bioassays. Rat IL-1 activity is neutralized incompletely. It does not neutralize the biological activity of mouse IL-1. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. Endotoxin Content: <10 pg/µl by LAL method. |
| Storage | Store vial at -20° C prior to opening. This product DOES NOT contain preservative. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening. |
| Research Area | Cytokines, Receptors, Inflammation |
| Restrictions | For Research Use only |
External Information: Interleukin-1 beta (IL-1-beta) (Mouse) antibody
| Google Scholar | Find more references for antigen Interleukin-1 beta (IL-1-beta) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Interleukin-1 beta (IL-1-beta) (Bioinformatic Harvester (EMBL)) |
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