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Application
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Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
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Purity
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
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Purification
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This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1R or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. Protein Sequence: Human Soluble Type II Interleukin-1 Receptor, 296 aa, predicted MW 33.6 kDa 1 MLRLYVLVMG VSAFTLQPAA HTGAARSCRF RGRHYKREFR LEGEPVALRC PQVPYWLWAS 61 VSPRINLTWH KNDSARTVPG EEETRMWAQD GALWLLPALQ EDSGTYVCTT RNASYCDKMS 121 IELRVFENTD AFLPFISYPQ ILTLSTSGVL VCPDLSEFTR DKTDVKIQWY KDSLLLDKDN 181 EKFLSVRGTT HLLVHDVALE DAGYYRCVLT FAHEGQQYNI TRSIELRIKK KKEETIPVII 241 SPLKTISASL GSRLTIPCKV FLGTGTPLTT MLWWTANDTH IESAYPGGRV TEGPRQ
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Storage
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Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.
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Research Area
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Cytokines, Receptors, Inflammation
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Restrictions
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For Research Use only
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