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Product details for 5-LO pSer523 (Human) antibody
5-LO pSer523 (Human) antibody
Overview
| Antigen: | Lipoxygenase (5-LO) pSer523 |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human, Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 340,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN152618 |
| Availability: | Ships in 7 to 10 days |
Additional Information: 5-LO pSer523 (Human) antibody
| Antigen | Lipoxygenase (5-LO) pSer523 |
| Immunogen | Phosphopeptide corresponding to amino acids residues surrounding the phosphoSer523 of human 5-Lipoxgenase (5-LO). |
| Reactivity | Human, Rat |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Description | The enzyme 5-lipoxygenase (5-LO) play a key role in regulating the production ofleukotrienes (LTs) (Funk, 2001). Overproduction of LTs contribute to several diseases,most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994)fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work hasdemonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine523in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may beimportant in limiting inflammation. Abnormal signaling through cAMP and PKA couldcontribute to a variety of diseases, including those characterized by chronicinflammation. The phospho-specific antibody to ser523 on 5-LO is thus likely to providea valuable tool for studies of the role of 5-LO regulation in diseases such as asthma,fibrosis, and atherosclerosis. Synonyms: 5-delta-lipoxygenase antibody, 5-lipoxygenase antibody, Arachidonic 5-lipoxygenaseantibody, Arachidonic acid 5-lipoxygenase antibody, C-5-lipoxygenase antibody,Delta(5)-lipoxygenase antibody, Leukotriene-A(4) synthase antibody, LTA synthaseantibody |
| Host | Rabbit |
| Specificity | Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots of rathippocampal lysate. Recognizes human and rat forms of 5-LO. Not tested in other species. |
Application Details: 5-LO pSer523 (Human) antibody
| Application | Western Blotting (WB) |
| Application Notes | This antibody has been tested in Western blot analysis. *Recommended starting dilution: Western blot - 1:1,000*The investigator should determine the optimal working dilution for a specific application. |
| Buffer | 0.1 ml affinity purified rabbit serum (affinity purification via sequential chromatographyon phospho- and non-phosphopeptide affinity columns). *Adequate amount of material to conduct 10-mini Western Blot. 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA |
| Storage | For long term storage -20 degrees Celcius is recommended. Stable at -20 degreesCelcius for at least 1 year. |
| Restrictions | For Research Use only |
External Information: 5-LO pSer523 (Human) antibody
| Google Scholar | Find more references for antigen Lipoxygenase (5-LO) pSer523 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Lipoxygenase (5-LO) pSer523 (Bioinformatic Harvester (EMBL)) |
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