Liv2 antibody

Overview


	Antigen:	Liv2
	Antibody Type:	Monoclonal
	Application:	Immunohistochemistry (IHC), Flow Cytometry (FACS)
	Host:	Rat
	Quantity:	100 µg (1 mg/mL)
	Price:	241,00 € (Plus shipping costs and VAT)
	Order number:	ABIN130630
	Availability:	7 to 10 days until shipment

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Additional Information: Liv2 antibody

Antigen	Liv2
Immunogen	This antibody was purified from hybridoma (clone Liv2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with WKY/Neij rat lymph nodes immunized with E11.5 murine fetal liver lysate.
Antibody Type	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	Liv2 is a fetal liver antigen that is expressed in murine embryos. Liv2 can be detected in fetal liver only during embryonic days 9.5 to 12.5, a period which corresponds to several key events in liver development. Nearly all cells in the hepatic bud were stained at E9.5, but the levels of Liv2 progressively decreased by E12.5, consistent with the numbers of hepatoblasts. Unlike other common fetal hepatic markers such as a-fetoprotein or albumin, Liv2 is not a diffusible serum protein but appears to be membrane specific. Thus, Liv2 is a useful tool for identifying individual murine hepatoblasts and for studying fetal liver development.
Clone	Liv2
Host	Rat
Specificity	This antibody reacts with Liv2.

Application Details: Liv2 antibody

Application	Immunohistochemistry (IHC), Flow Cytometry (FACS)
Application Notes	Western blotting: Not tested. Immunohistochemistry: 5-20 µg / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). Flow Cytometry: 10 µg/mL (final concentration)
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Restrictions	For Research Use Only

Publications: Liv2 antibody

Publications

Watanabe, Nakagawa, Ohata et al.:
"SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis."
In: Developmental biology , Vol. 250, Issue 2, pp. 332-47, 2002/01 (PubMed)

Images: Liv2 antibody

Immunohistochemical detection of Liv2 on paraffin embedded section of mouse embryonic liver with ABIN130630.

Image for Liv2 antibody (1)

External Information: Liv2 antibody

Google Scholar	Find more references for clone Liv2 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Liv2 (Bioinformatic Harvester (EMBL))