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Application
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Enzyme Immunoassay (EIA), Western Blotting (WB)
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Application Notes
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Immunoblotting: 1.0 ug/ml for AP/BCIP/NBT (MTT) detection, 0.5 ug/ml for HRP/ECL detection ELISA: use at 0.1-1.0 ug/ml Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
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Concentration
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100 ug/ml (prior to lyophilization)
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Buffer
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This antibody is lyophilized from thiophilic adsorption and size exclusion chromatography purified Ig fraction in 2X PBS, PEG and sucrose, containing 0.09 % sodium azide as preservative. Reconstitute with 1ml water (15 minutes at room temperature).
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Storage
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Store lyophilized product at –20 °C. After reconstitution, aliquot and store at –80 °C. Thawed aliquots may be stored for up to 3 months at 2-8 °C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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