NK Cells (CD16/56) FITC (Human) antibody

Overview

Antigen	NK Cells (CD16/56)
Immunogen	U266 myeloma cell line.
Reactivity	Human
Antibody Type	Monoclonal
Isotype	Cocktail »Matching secondary antibodies
Clone	LNK16-MEM-188
Host	Mouse
Specificity	This antibody is a combination of two monoclonal antibodies recognising the human CD16 and CD56 cell surface antigens. This combination is particularly useful for the detection of all natural killer cells due to the fact that different subsets of these cells exist that are either CD16+/CD 56+, CD16+/CD56- or C D16-/C D56+.

Application	Flow Cytometry (FACS)
Application Notes	Flow Cytometry: neat – 1/10, use 10ul of suggested working dilution to label 106 cells in 100ul. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Conjugate	FITC
Concentration	0.1 mg/ml
Buffer	This antibody is supplied as liquid, Protein G affinity purified IgG fraction, conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) in Phosphate buffered saline pH 7.2 with 0.09% sodium azide as preservative and 1% BSA as stabilizer.
Storage	Store the antibody at 4-8°C for one month or at –20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: 18 months from despatch.
Restrictions	Research only

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