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Product details for NMDA NR1 Pan (Rat) antibody
NMDA NR1 Pan (Rat) antibody
Overview
| Antigen: | NMDA Receptor 1 (NR1) Pan |
| Antibody Type: | Monoclonal |
| Application: | Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 350,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN152467 |
| Availability: | Ships in 7 to 10 days |
Additional Information: NMDA NR1 Pan (Rat) antibody
| Antigen | NMDA Receptor 1 (NR1) Pan |
| Immunogen | NB 300-118 was raised against the fusion protein from amino acids 1 - 564 of NMDA NR1. |
| Reactivity | Rat |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG »Matching secondary antibodies |
| Description | The ion channels activated by glutamate are divided into two classes. Those that aresensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR)while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionicacid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).NMDA receptors are among the most studied receptors in neuroscience because theyare involved in neuronal cell development and plasticity, a cellular correlate for learning.NMDA receptors are also implicated in several disorders of the central nervous systemincluding epilepsy and ischemic neuronal cell death. NMDA receptors also appear to bea target for ethanol at physiological concentrations and therefore may play a significantrole in alcoholism. |
| Clone | R1JHL |
| Host | Mouse |
| Specificity | NB 300-118 is specific to NMDA NR1. The specificity is defined based on NB 300-118'sspecific immunolabeling of the 120 kDa band in Western blots of rat hippocampalhomogenate at an antibody dilution of 1:300. ABIN152467 can be used for detection of NMDA in all NR1 variants in rat. Other specieshave not been tested. |
Application Details: NMDA NR1 Pan (Rat) antibody
| Application | Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | NB 300-118 can be used in Western blot where a band is seen at ~ 120 kDarepresenting NMDA NR1.Suggested working dilutions: *Western Blot 1:1000**Immunoprecipitation 3ug/200ug lysate*The investigator should determine the optimal dilution for a specific application.**For immunoblot, use in TBST (20 mM Tris/HCl (pH 7.6), 137 mM NaCl, 1% Tween-20)containing 5% non-fat milk and 0.05% Sodium azide. This solution may be used at least5 times without loss in signal strength. Positive Control:Rat hippocampal homogenate. |
| Concentration | 0.3 mg/ml |
| Buffer | 15 ug of affinity purified anti-NMDA NR1 lyophilized. Reconstitute in 50 ul of distilledwater. Tissue culture supernatant |
| Storage | After reconstitution store at –20°C. Avoid repeated freeze-thaw cycles. |
| Restrictions | For Research Use only |
External Information: NMDA NR1 Pan (Rat) antibody
| Google Scholar | Find more references for clone R1JHL on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen NMDA Receptor 1 (NR1) Pan (Bioinformatic Harvester (EMBL)) |
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