Neuraminidase (Neu1) (Human) antibody

Overview


	Antigen:	Neuraminidase (Neu1)
	Antibody Type:	Polyclonal
	Application:	Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	0.2 ml (85 mg/ml (by Refractometry))
	Price:	340,00 € (Plus shipping costs and VAT)
	Order number:	ABIN117991
	Availability:	Ships in 3 to 10 days

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Additional Information: Neuraminidase (Neu1) (Human) antibody

Antigen	Neuraminidase (Neu1)
Immunogen	This whole rabbit serum was prepared by repeated immunizations with a bacterially expressed and purified fusion protein excluding the signal peptide (first 45 amino acids) and the first 6 N-terminal amino acids of the processed form of human lysosomal neuraminidase (see link below for the full length sequence of the human gene product).
Reactivity	Human
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit

Application Details: Neuraminidase (Neu1) (Human) antibody

Application	Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. This product was assayed on immunoblots against human primary fibroblast cell lysates. A single band of the expected apparent molecular weight was observed at a 1:500 dilution incubated for 2 h at room temperature. For immunofluorescence microscopy a 1:500 dilution is recommended incubated for 2 h at room temperature or alternatively, a 1:1,000-1:5,000 dilution can be used incubated overnight at room temperature. Staining is best on paraformaldehyde-fixed cultures. The optimal dilution and time of incubation is dependent on the methods used, the type of cells or tissue, and the amount of protein present. Neuraminidase is not very abundant in most tissues and its detection with the antibodies may require further optimization. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Purity and Specificity: This antiserum is directed against Neu1 and reacts with the Neu1 from human tissues. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. WT GS-K IB AF SG SL RD IL EV GV GMO Figure 1. Western blot analysis using anti-Neu1 antibody to detect neuraminidase in cell lysates (5 ug each) of fibroblasts from patients. Anti-Neu1 (top) and anti-PPCA antibodies (bottom) were used to analyze western blots containing protein (5 ug) from fibroblast lysates of a healthy person (WT), five patients with type I sialidosis and five patients with type II sialidosis. This antibody detects 46 kDa neuraminidase. Communicated with permission by E. Bonten. See Bonten et al (2000) for additional details. 46 kDa a-neur a-PPCA 32 kDa
Concentration	85 mg/ml (by Refractometry)
Buffer	This purified antibody was liquid (sterile filtered), IgG fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.09% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C or belo w prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.
Research Area	Enzymes, Metabolism
Restrictions	Research only

Images: Neuraminidase (Neu1) (Human) antibody

Western blot analysis using anti-Neu1 antibody to detect neuraminidase in cell lysates (5 ug each) of fibroblasts from patients. Anti-Neu1 (top) and anti-PPCA antibodies (bottom) were used to analyze western blots containing protein (5 ug) from fibroblast lysates of a healthy person (WT), five patients with type I sialidosis and five patients with type II sialidosis. This antibody detects 46 kDa neuraminidase. Communicated with permission by E. Bonten. See.,.Bonten et al (2000) for additional details.

Image for Neuraminidase (Neu1) (Human) antibody (1)

Immunocytochemical localization using.,.anti-Neu1 antibody to detect neuraminidase in cell lysates of fibroblasts from a healthy person (WT and patient 7 (see above). Anti-Neu1 antibodies were used in.,.conjunction with FITC-conjugated Gt-a-Rabbit IgG. The nuclei were stained with DAPI. The magnification is 400x. Communicated with permission by E. Bonten. See Bonten et al(2000) for additional details.

Image for Neuraminidase (Neu1) (Human) antibody (2)

Immunocytochemical localization of neuraminidase.,.overexpressed in sialidosis fibroblasts. Sialidosis fibroblasts.,.were either transfected with the wild-type PPCA and.,.neuraminidase cDNA expression constructs (WT) or with the.,.wild-type PPCA and mutant neuraminidase cDNA expression.,.plasmids as indicated. Cells were seeded on slides and.,.incubated with anti-Neu1 antibodies and FITC-conjugated.,.Gt-a-Rabbit IgG. and FITC-conjugated secondary antibodies. The nuclei were stained with DAPI. The magnification is 400x. Communicated with permission by E. Bonten. See Bonten et al (2000) for additional details.

Image for Neuraminidase (Neu1) (Human) antibody (3)

External Information: Neuraminidase (Neu1) (Human) antibody

Google Scholar	Find more references for antigen Neuraminidase (Neu1) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Neuraminidase (Neu1) (Bioinformatic Harvester (EMBL))