Neuraminidase (Neu2) (Human) antibody

Overview


	Antigen:	Neuraminidase (Neu2)
	Antibody Type:	Polyclonal
	Application:	Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	0.1 mg (0.9 mg/ml (by UV absorbance at...)
	Price:	340,00 € (Plus shipping costs and VAT)
	Order number:	ABIN117994
	Availability:	Ships in 3 to 10 days

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Additional Information: Neuraminidase (Neu2) (Human) antibody

Antigen	Neuraminidase (Neu2)
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-124 of Human Neu2.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody reacts with human Neu2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established. Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2. Peptide sequence: T-E-Q-Q-Q-L-Q-T-R-A-N-V-T-R-L

Application Details: Neuraminidase (Neu2) (Human) antibody

Application	Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. This product was assayed by ELISA against 0.1 ug of the immunizing peptide. A 1:2,000 to 1:10,000 dilution of the antibody is recommended for this assay. This product was assayed on immunoblot against both recombinant protein and an E.coli lysate expressing Neu-2. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications. 43kDaFigure 1. Western blot analysis using Rockland Immunochemical's Affinity Purifiedanti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ug loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™ 800 labeled Goat-a-Rabbit IgG [H&,L] diluted 1:1,000. IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Background information: Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of a- glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetylneuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with 1human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.
Concentration	0.9 mg/ml (by UV absorbance at 280 nm)
Buffer	This antibody was lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
Research Area	Enzymes, Metabolism
Restrictions	Research only

Publications: Neuraminidase (Neu2) (Human) antibody

Publications

Fanzani, Giuliani, Colombo et al.:
"Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells."
In: FEBS letters , Vol. 547, Issue 1-3, pp. 183-8, 2003/01 (PubMed)

Tringali, Papini, Fusi et al.:
"Properties of recombinant human cytosolic sialidase HsNEU2. The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules."
In: The Journal of biological chemistry , Vol. 279, Issue 5, pp. 3169-79, 2004/01 (PubMed)

Images: Neuraminidase (Neu2) (Human) antibody

Western blot analysis using.,.Rockland Immunochemical's Affinity Purified.,.anti-Neu2 antibody to detect recombinant.,.His tagged Neu-2 (1.0 ug loaded)..,.Molecular weight marker (not shown).,.indicates a single band of the expected MW.,.(43 kDa). The blot was incubated with a.,.1:500 dilution of the antibody at room.,.temperature for 1 h followed by detection.,.using IRDye™800 labeled Goat-a-Rabbit.,.IgG [H&L] diluted 1:1,000..,.IRDye™800 fluorescence image was.,.captured using the Odyssey® Infrared.,.Imaging System developed by LI-COR..,.IRDye is a trademark of LI-COR, Inc. Other.,.detection systems will yield similar results.

Image for Neuraminidase (Neu2) (Human) antibody (1)

Western blot analysis using Affinity Purified.,.anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2 (1.0 ul loaded). Molecular weight marker (not shown) indicates a band of the expected.,.MW (43 kDa). The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the.,.antibody at room temperature for 1 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:1,000. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark.,.of LI-COR, Inc. Other detection systems will yield similar results.

Image for Neuraminidase (Neu2) (Human) antibody (2)

External Information: Neuraminidase (Neu2) (Human) antibody

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