Neurochondrin (Human) antibody

Overview

Antigen	Neurochondrin
Gene ID	23154 , 26562 (mouse) , 89791 (rat)
Immunogen	Peptide with sequence C-HYLLQVGSEKQKEP, from the internal region of the protein sequence according to NP_055099.1, NP_001014839.1, NP_001014841.1
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: NCDN Accession Number(s): NP_055099.1, NP_001014839.1, NP_001014841.1. . Principal Names: NCDN, neurochondrin , KIAA0607.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 100kDa and140kDa in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.9kDa according to NP_055099.1 and 77.2kDa according to NP_001014841.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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