Neutrophils (GM3.2) (Mouse) antibody

Overview

Antigen	Neutrophils (GM3.2)
Immunogen	B6.Ly-1 peritoneal exudate cells . Fusion Partner: P3-NS1-Ag4 (NS-1) . Donor: 129/ReJ mouse spleen
Reactivity	Mouse (Murine)
Antibody Type	Monoclonal
Format	Ascitic fluid (lyophilized)
Isotype	IgG2a »Matching secondary antibodies
Description	Anti-mouse neutrophils (GM3.2) monoclonal antibody defines a unique mouse neutrophil cell surface antigen named GM3.2 (GM-3 locus, granulocyte- macrophage antigen-3, Chr 2). This phenotypic specific antigen is present only on strains of mice expressing the GM3.2 phenotype (A/WySn, C57BL/6, C57BL/10, C57BR/cd, C57L,J, RF/J are GM3.2 +'ve and BALB/c, C3H/He, AKR are examples of GM3.2 -'ve strains). GM3.2 is found on neutrophils in bone marrow, peritoneal exudates and a population of thioglycollate-activated macrophages.(1) It is absent from lymphoid, kidney liver, heart and red cells. GM3.2 should prove to be a useful marker for studies of myeloid cell differentiation, as granulocyte/macrophage colony forming cells are GM3.2 negative, while mature neutrophils and activated macrophages are GM3.2 positive. (2)
Host	Mouse
Specificity	Mouse Neutrophils (GM3.2)

Application	Flow Cytometry (FACS)
Buffer	Ascitic fluid (lyophilized)
Storage	Lyophilized form stable at 4°C or -20°C. Reconstitute with 0.5 ml of cold distilled water. After reconstitution, aliquot and freeze unused portions in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
Research Area	Immunology, Cell/Tissue Markers
Restrictions	For Research Use only

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