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Product details for Nitrotyrosine antibody - Biotinylated
Nitrotyrosine antibody - Biotinylated
Overview
| Antigen: | Nitrotyrosine - Biotinylated |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) |
| Host: | Mouse |
| Quantity: | |
| Price: | 256,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN110293 |
| Availability: | 1 to 7 days before shipment |
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Additional Information: Nitrotyrosine antibody - Biotinylated
| Antigen | Nitrotyrosine - Biotinylated |
| Immunogen | Peroxynitrite-treated KLH |
| Antibody Type | Monoclonal |
| Description | Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by NO synthase. NO reacts rapidly with superoxide (6.7 x 109 M-1sec-1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. Cayman Chemical's biotinylated nitrotyrosine monoclonal antibody can be used for the species-independent detection of nitrotyrosine using a variety of immunochemical techniques. Biotinylation of the antibody allows for detection using avidin-enzyme or avidin-fluorophore conjugates and provides approximately 2-fold better sensitivity compared to the unbiotinylated antibody (Catalog No. 189542). |
| Host | Mouse |
| Specificity | Species independent detection of nitrotyrosine: +, chlorotyrosine: <5% |
Application Details: Nitrotyrosine antibody - Biotinylated
| Application | Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) |
| Buffer | 100 µg purified IgG2b-biotin in 200 µl PBS, pH 7.2, containing 0.5 mg/ml BSA and 0.02% sodium azide |
| Storage | 1 year |
| Restrictions | For Research only |
External Information: Nitrotyrosine antibody - Biotinylated
| Google Scholar | Find more references for antigen Nitrotyrosine - Biotinylated on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Nitrotyrosine - Biotinylated (Bioinformatic Harvester (EMBL)) |
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