Product details for Occludin (1A8) (Rat) antibody
Occludin (1A8) (Rat) antibody
Overview
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| Antigen: | Occludin (1A8) |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (IHC), Western Blotting (WB) |
| Reactivity: | Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 315,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN180065 |
| Availability: | 10 days until shipment |
Additional Information: Occludin (1A8) (Rat) antibody
| Antigen | Occludin (1A8) |
| Immunogen | Recombinant Rat Occludin |
| Reactivity | Rat |
| Antibody Type | Monoclonal |
| Isotype | IgM »Matching secondary antibodies |
| Description | Occludin is an approximately 60kDa-integral membrane protein localizing at Tight-Junction, and composed of short N-terminal cytosolic domain, four transmembrane domains and long C-terminal cytosolic domain. It is reported that Occludin has no homology to claudin family and forms strand by coporimerization with claudin family proteins. It is considered that Tight-Junction plays an important role in maintenance of cellular polarity (fence function) and in regulating the passage of ions and small molecular through the paracellular pathway (barrier function). This antibody can be used to stain the formalin-fixed paraffin-embedded sections in immunohistochemistry. |
| Clone | 1A8 |
| Host | Mouse |
| Specificity | Cross-reacts with Human and Dog |
Application Details: Occludin (1A8) (Rat) antibody
| Application | Immunohistochemistry (IHC), Western Blotting (WB) |
| Application Notes | This antibody can be stained in formalin fixed paraffin embedded tissues after autoclave pretreatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is about 0.5 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of about 0.1~0.5 ug/ml. |
| Purification | Anti-mouse IgM |
| Buffer | Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 50 ug/ml |
| Storage | Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20 |
| Restrictions | For Research Use only |








