RAB11FIP1 (Human) antibody

Overview

Antigen	RAB11FIP1 (internal)
Gene ID	80223
Immunogen	Peptide with sequence C-EATKEAKESKKPE, from the internal region of the protein sequence according to NP_079427.3, NP_001002814.1
Reactivity	Human
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: RAB11FIP1 Accession Number(s): NP_079427.3, NP_001002814.1. . Principal Names: RAB11FIP1, RAB11 family interacting protein 1 (class I) , HGNC:30265, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1 , Rab coupling protein, Rab effector protein, Rab-interacting recycling protein, Rab11-family interacting protein 1D, no Rab11-binding protein 1A.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human

Application Notes	Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 110kDa, 50kDa and 38kDa in Human Breast lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 71.0kDa according to NP_079427.3, and 137kDa according to NP_001002814.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Please note that an additional 55kDa was only partly blocked by the immunizing peptide, this band may not be specific. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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