RIZ antibody

Overview


	Antigen:	RIZ
	Application:	Immunocytochemistry (ICC), Immunoprecipitation (IP)
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	266,00 € (Plus shipping costs and VAT)
	Order number:	ABIN131766
	Availability:	7 to 10 days until shipment

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Additional Information: RIZ antibody

Antigen	RIZ
Immunogen	This antibody was purified from hybridoma (clone RIZ1723) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with human recombinant RIZ (1-618a.a.).
Antibody Type
Isotype	IgG2b »Matching secondary antibodies
Description	RIZ1 (Retinoblastoma protein- interacting zinc finger), also known as PRDM2, is a nuclear protein containing 8 zinc finger motifs. RIZ1 contains a PR domain that may function as protein binding interface in the regulation of chromatin-mediated gene expression. RIZ 1 acts as a tumor suppressor, and RIZ 1 inactivation is common in many types of human cancers, including neuroendocrine, breast, liver, colon, skin, and lymphoid tumors. Similarly, RIZ1 over-expression in breast cancer cells caused cell cycle arrest in G2-M and apoptosis. RIZ1 is highly expressed in human retinoblastoma cells and at low levels in all other human cell lines.
Clone	RIZ1723
Host	Mouse
Specificity	This antibody immunoprecipitates human RIZ (220kDa).

Application Details: RIZ antibody

Application	Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes	Western blotting: Not recommended. Immunohistochemistry: Not recommended. Immunocytochemistry: 2µg/mL. Immunoprecipitation: 10µg / 200µL of cell extract from 5x10 6 cells. Flow cytometry: Not tested.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.
Restrictions	For Research Use Only

Publications: RIZ antibody

Publications

Abbondanza, Medici, Nigro et al.:
"The retinoblastoma-interacting zinc-finger protein RIZ is a downstream effector of estrogen action."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 97, Issue 7, pp. 3130-5, 2000/01 (PubMed)

He, Yu, Liu et al.:
"RIZ1, but not the alternative RIZ2 product of the same gene, is underexpressed in breast cancer, and forced RIZ1 expression causes G2-M cell cycle arrest and/or apoptosis."
In: Cancer research , Vol. 58, Issue 19, pp. 4238-44, 1998/01 (PubMed)

Steele-Perkins, Fang, Yang et al.:
"Tumor formation and inactivation of RIZ1, an Rb-binding member of a nuclear protein-methyltransferase superfamily."
In: Genes & development , Vol. 15, Issue 17, pp. 2250-62, 2001/01 (PubMed)

Images: RIZ antibody

Immunoprecipitation of RIZ from MCF7 cells with mouse IgG2b (1) or ABIN131766 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDSPAGE and immunoblotted with ABIN131766.

Image for RIZ antibody (1)

External Information: RIZ antibody

Google Scholar	Find more references for clone RIZ1723 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen RIZ (Bioinformatic Harvester (EMBL))