UBE2E3 (Human) antibody

Overview

Antigen	UBE2E3 / UBCH9
Gene ID	10477 , 22193 (mouse)
Immunogen	Peptide with sequence SSDRQRSDDESPS-C, from the N Terminus of the protein sequence according to NP_006348.1, NP_872619.1
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: UBE2E3 Accession Number(s): NP_006348.1, NP_872619.1 Gene Ontology terms: ubiquitin conjugating enzyme activity, ubiquitin-protein ligase activity, ubiquitin cycle, protein modification, ligase activity Both reported variants represent identical protein (NP_006348.1, NP_872619.1).. . Principal Names: UBE2E3, ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast), UBCH9, UbcH9, UbcM2, ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5), ubiquitin carrier protein.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application Notes	Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: No signal obtained yet but low background observed in Human Brain and Human Testis lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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EMBL Harvester	Search human, mouse or rat genome for antigen UBE2E3 / UBCH9 (Bioinformatic Harvester (EMBL))