NCE2 (Human) antibody

Overview

Antigen	UBE2F / NCE2
Gene ID	140739
Immunogen	Peptide with sequence C-RNKVDDYIKRYAR, from the C Terminus of the protein sequence according to NP_542409
Reactivity	Human, Mouse (Murine), Xenopus
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: UBE2F Accession Number(s): NP_542409 Gene Ontology terms: ligase activity, ubiquitin conjugating enzyme activity, ubiquitin cycle. . Principal Names: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human

Application Notes	Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

Google Scholar	Find more references for antigen UBE2F / NCE2 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen UBE2F / NCE2 (Bioinformatic Harvester (EMBL))